In vitro probiotic characteristics of Lactobacillus plantarum ZDY 2013 and its modulatory effect on gut microbiota of mice
In vitro probiotic characteristics of Lactobacillus plantarum ZDY 2013 and its modulatory effect on gut microbiota of mice
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植物乳杆菌ZDY 2013的体外益生特性及其对小鼠肠道菌群的调节作用
DOI：
10.3168/jds.2014-9153
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发表时间：
2015-09-01
影响因子：
3.5
通讯作者：
Wei, Hua
中科院分区：
文献类型：
--
作者：
Huang, Renhui;Tao, Xueying;Wei, Hua
从中国传统发酵酸豆中分离到一株新的植物乳杆菌（Lactobacillus plantarum ZDY 2013），对其在pH、胆盐、模拟胃肠道、抗生素等逆境条件下的存活能力、胞外多糖的产生以及对8种病原菌的拮抗作用进行了系统评价。采用定量PCR和PCR-变性梯度凝胶电泳技术研究其对小鼠肠道菌群的影响。结果表明，ZDY 2013在pH3.5条件下生长，在pH2.0条件下存活6h，在0.45%胆盐条件下存活3h。胞外多糖产量高达204 ± 7.68 mg/L。ZDY 2013在模拟胃肠道中的存活率高达65.84%。用ZDY 2013的上清液进行的拮抗试验显示对单核细胞增生李斯特菌的最大晕圈为28 mm。抑制效果依次为：单核细胞增生李斯特菌、鼠伤寒沙门氏菌、大肠埃希菌、铜绿假单胞菌、宋内志贺菌、坂崎肠杆菌、金黄色葡萄球菌。植物乳杆菌ZDY 2013对一些抗生素敏感（例如，大环内酯类、磺胺类、氨基糖苷类、四环素类和β-内酰胺类），而对糖肽类、喹诺酮类和头孢菌素类抗生素耐药。变性梯度凝胶电泳图谱表明，ZDY 2013给药改变了小鼠各种肠道位点的微生物群组成。此外，定量PCR测试显示，施用ZDY 2013增强了结肠或盲肠中双歧杆菌和乳杆菌的群体，并减少了潜在的肠道致病性细菌（例如，肠球菌、肠杆菌和产气荚膜梭菌）。植物乳杆菌ZDY 2013对低pH值、胆盐和胃肠液具有很高的耐受性，并具有抗菌和肠道微生物群调节特性，在乳制品和保健品开发中具有潜在的应用前景。
Lactobacillus plantarum ZDY 2013, a novel strain isolated from Chinese traditional fermented acid beans, was systematically evaluated for its survival capacity under stress conditions (pH, bile salt, simulated gastrointestinal tract, and antibiotics), production of exopolysaccharide and antagonism against 8 pathogens. Its effect on mice gut microbiota was also investigated by quantitative PCR and PCR-denaturing gradient gel electrophoresis. The results showed that ZDY 2013 can grow at pH 3.5 and survive at pH 2.0 for 6 h and at 0.45% bile salt for 3 h. The exopolysaccharide yield was up to 204 +/- 7.68 mg/L. The survival rate of ZDY 2013 in a simulated gastrointestinal tract was as high as 65.84%. Antagonism test with a supernatant of ZDY 2013 showed maximum halo of 28 mm against Listeria monocytogenes. The inhibition order was as follows: Listeria monocytogenes, Salmonella typhimurium, Escherichia coli, Pseudomonas aeruginosa, Shigella sonnei, Enterobacter sakazakii, and Staphylococcus aureus. Lactobacillus plantarum ZDY 2013 was sensitive to some antibiotics (e.g., macrolide, sulfonamides, aminoglycoside, tetracyclines and P-lactams), whereas it was resistant to glycopeptides, quinolones, and cephalosporins antibiotics. Denaturing gradient gel electrophoresis profile demonstrated that ZDY 2013 administration altered the composition of the microbiota at various intestinal loci of the mice. Moreover, the quantitative PCR test showed that the administration of ZDY 2013 enhanced the populations of Bifidobacterium and Lactobacillus in either the colon or cecum, and reduced the potential enteropathogenic bacteria (e.g., Enterococcus, Entero bacterium, and Clostridium perfringens). Lactobacillus plantarum ZDY 2013 exhibited high resistance against low pH, bile salt, and gastrointestinal fluid, and possessed antibacterial and gut microbiota modulation properties with a potential application in the development of dairy food and nutraceuticals.