A BCAM0223 mutant of Burkholderia cenocepacia is deficient in hemagglutination, serum resistance, adhesion to epithelial cells and virulence.
A BCAM0223 mutant of Burkholderia cenocepacia is deficient in hemagglutination, serum resistance, adhesion to epithelial cells and virulence.
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DOI：
10.1371/journal.pone.0041747
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Fialho AM
中科院分区：
文献类型：
--
作者：
Mil-Homens D;Fialho AM
洋葱伯克霍尔德氏菌复合体（Bcc）细菌是一组有问题的微生物，可导致囊性纤维化患者严重感染。在感染的早期阶段，BCC细菌必须能够粘附并定植于呼吸道上皮。虽然这还不完全清楚，但据信感染的初级阶段部分是由一种特定类型的粘附素介导的，称为三聚体自转运粘附素（TAAs）。这些同源三聚体蛋白存在于许多革兰氏阴性病原体的表面上，并且通常介导许多关键功能，包括生物膜形成、血清抗性和对宿主细胞入侵的粘附。我们先前已在流行性临床分离株B的基因组中鉴定出。cenocepacia J2315，一个新的编码三种TAA（BCAM 0219，BCAM 0223和BCAM 0224）的puerectin基因簇。在这项研究中，TAA簇的基因组组织已经确定。为了进一步阐明推定的TAA BCAM 0223在B中的直接作用。新洋葱致病性，通过插入失活构建了一个同基因突变体。BCAM 0223：：Tp突变体缺乏血凝作用，影响玻连蛋白粘附和生物膜形成，并在大蜡螟感染模型中表现出减弱的毒力。此外，BCAM 0223：：Tp突变体还显示出其对人血清的抗性以及对培养的人支气管上皮细胞的粘附性显著降低，但对人支气管上皮细胞的侵袭性没有降低。总之，这些结果表明，BCAM 0223蛋白是一个多功能的毒力因子，可能有助于B的致病性。cenocepacia。
Burkholderia cepacia complex (Bcc) bacteria are a problematic group of microorganisms causing severe infections in patients with Cystic Fibrosis. In early stages of infection, Bcc bacteria must be able to adhere to and colonize the respiratory epithelium. Although this is not fully understood, this primary stage of infection is believed to be in part mediated by a specific type of adhesins, named trimeric autotransporter adhesins (TAAs). These homotrimeric proteins exist on the surface of many Gram negative pathogens and often mediate a number of critical functions, including biofilm formation, serum resistance and adherence to an invasion of host cells. We have previously identified in the genome of the epidemic clinical isolate B. cenocepacia J2315, a novel cluster of genes putatively encoding three TAAs (BCAM0219, BCAM0223 and BCAM0224). In this study, the genomic organization of the TAA cluster has been determined. To further address the direct role of the putative TAA BCAM0223 in B. cenocepacia pathogenicity, an isogenic mutant was constructed via insertional inactivation. The BCAM0223::Tp mutant is deficient in hemagglutination, affected in adherence to vitronectin and in biofilm formation and showed attenuated virulence in the Galleria mellonella model of infection. Moreover, the BCAM0223::Tp mutant also showed a significant reduction in its resistance to human serum as well as in adherence, but not in invasion of, cultured human bronchial epithelial cells. Altogether these results demonstrate that the BCAM0223 protein is a multifunctional virulence factor that may contribute to the pathogenicity of B. cenocepacia.
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影响因子：
4.4
作者：
Hallstrom, Teresia;Trajkovska, Elena;Riesbeck, Kristian
通讯作者：
Riesbeck, Kristian
影响因子：
3.1
作者：
Elkins, C;Morrow, KJ;Olsen, B
通讯作者：
Olsen, B
DOI：
10.1073/pnas.0507749103
发表时间：
2006-02-28
影响因子：
11.1
作者：
Heise, T;Dersch, P
通讯作者：
Dersch, P
影响因子：
14.9
作者：
Finn RD;Mistry J;Tate J;Coggill P;Heger A;Pollington JE;Gavin OL;Gunasekaran P;Ceric G;Forslund K;Holm L;Sonnhammer EL;Eddy SR;Bateman A
通讯作者：
Bateman A
影响因子：
5.1
作者：
Bruscia, E;Sangiuolo, F;Gruenert, DC
通讯作者：
Gruenert, DC