Excretion profiles of ethyl glucuronide in human urine after internal dilution
Excretion profiles of ethyl glucuronide in human urine after internal dilution
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DOI：
10.1093/jat/26.5.262
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发表时间：
2002-07-01
影响因子：
2.5
通讯作者：
Aderjan, RE
中科院分区：
文献类型：
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作者：
Goll, M;Schmitt, G;Aderjan, RE
葡萄糖醛酸乙酯（EG）是一种有用的酒精消耗标记物，因为它的存在可以在尿液中检测到长达5天。我们研究了利尿对尿中微量乙醇代谢物FG排泄的影响。7名健康志愿者在15分钟内喝了250毫升葡萄酒（25克乙醇），240分钟后，在15分钟内喝了1升水。开始饮酒前排尿，此后每30-60分钟排尿一次，持续400-550分钟。尿乙基葡萄糖醛酸（UEG）、肌酐和乙醇分别采用液相色谱-串联质谱法、jaff<s:1>法和酶促ADH法测定。最大利尿与饮酒后250 ~ 400 min UEG最低浓度2 mg/L和肌酐最低浓度10 mg/dL一致。饮酒后尿肌酐下降缓慢。在短暂的增加后，由于取水的原因，它下降到最小值。入水1 L后，利尿作用在60 min内增加至最大。尿中葡萄糖醛酸乙酯排泄量为10 mg (SD 5 mg)，相当于给药剂量的0.04% （SD 0.02%）。在排空期的连续排空中，受试者饮用1 L水后，UEG和利尿均受到影响。最小UEG值仍可测到0.5 mg/L。测量UEG是监测近期饮酒情况的可靠方法。然而，尿肌酐需要额外测量。建议将稀释样品中尿肌酐的临界值设定为25 mg/dL，如用于非法药物的分析。如果肌酐值太低，分析员必须决定是否进行进一步的检查。
Ethyl glucuronide (EG) is a useful marker of alcohol consumption because its presence in urine can be detected up to five days. We investigated the impact of diuresis on the urinary excretion of FG, a minor ethanol metabolite. Seven healthy volunteers drank 250 mL of wine (25 g ethanol) in 15 min and, 240 min later, ingested 1 L of water within 15 min. Urine was voided before the drinking started and every 30–60 min for 400–550 min thereafter. Urinary ethyl glucuronide (UEG), creatinine, and ethanol were determined using liquid chromatography-tandem mass spectrometry, Jaffé's method, and the enzymatic ADH method, respectively. The maximum diuresis coincided with the lowest values of the UEG concentrations of 2 mg/L and the lowest creatinine values of 10 mg/dL 250–400 min after drinking. After drinking the wine, the urinary creatinine decreased slowly. After a short period of increasing, it decreased to minimum values caused by the water intake. After the intake of 1 L water, the diuresis increased within 60 min to its maximum. The amount of ethyl glucuronide excreted in urine was 10 mg (SD 5 mg) corresponding to 0.04% (SD 0.02%) of the dose administered. In successive voids during the elimination phase, the UEG and the diuresis were influenced after the subjects drank 1 L of water. Minimum UEG values of 0.5 mg/L could still he measured. Measuring UEG provides a reliable way to monitor recent drinking of alcohol. However, urinary creatinine needs to be measured additionally. Establishing a cutoff value of 25 mg/dL for urinary creatinine in diluted samples, like for the analysis of illicit drugs, is recommended. If the creatinine value is too low, the analyst has to decide about the further procedure.