Development and Validation of Indirect Enzyme-Linked Immunosorbent Assays for Detecting Antibodies to SARS-CoV-2 in Cattle, Swine, and Chicken.
Development and Validation of Indirect Enzyme-Linked Immunosorbent Assays for Detecting Antibodies to SARS-CoV-2 in Cattle, Swine, and Chicken.
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间接酶联免疫吸附剂的开发和验证，用于检测牛，猪和鸡肉中SARS-COV-2的抗体。
DOI：
10.3390/v14071358
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发表时间：
2022-06-22
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多种家畜和野生动物物种对SARS-CoV-2感染易感。牛和猪对实验性SARS-CoV-2感染易感。SARS-CoV-2在动物宿主中不受控制的传播可能导致病毒适应和新变体的出现。此外，SARS-CoV-2在牲畜中的扩散和随后的适应可能会严重影响粮食安全以及动物和公共卫生。因此，监测SARS-CoV-2对牲畜的传播至关重要。我们开发并优化了种特异性间接ELISA（iELISA），以检测牛，猪和鸡中的抗SARS-CoV-2抗体，使用刺突蛋白受体结合域（RBD）抗原。于2019冠状病毒病大流行前收集的血清样本用于确定临界值。将来自牛（n = 3）、猪（n = 6）和鸡（n = 3）的RBD超免疫血清用作阳性对照。使用COVID-19大流行期间采集的牛（n = 150）、猪（n = 150）和鸡（n = 150）血清样本，与活病毒中和试验进行比较，对iELISA进行了评价。牛、猪和鸡的iELISA具有100%的灵敏度和特异性。这些工具有助于进行必要的监测，以迅速查明对全球三个最重要农业物种的溢出效应。
Multiple domestic and wild animal species are susceptible to SARS-CoV-2 infection. Cattle and swine are susceptible to experimental SARS-CoV-2 infection. The unchecked transmission of SARS-CoV-2 in animal hosts could lead to virus adaptation and the emergence of novel variants. In addition, the spillover and subsequent adaptation of SARS-CoV-2 in livestock could significantly impact food security as well as animal and public health. Therefore, it is essential to monitor livestock species for SARS-CoV-2 spillover. We developed and optimized species-specific indirect ELISAs (iELISAs) to detect anti-SARS-CoV-2 antibodies in cattle, swine, and chickens using the spike protein receptor-binding domain (RBD) antigen. Serum samples collected prior to the COVID-19 pandemic were used to determine the cut-off threshold. RBD hyperimmunized sera from cattle (n = 3), swine (n = 6), and chicken (n = 3) were used as the positive controls. The iELISAs were evaluated compared to a live virus neutralization test using cattle (n = 150), swine (n = 150), and chicken (n = 150) serum samples collected during the COVID-19 pandemic. The iELISAs for cattle, swine, and chicken were found to have 100% sensitivity and specificity. These tools facilitate the surveillance that is necessary to quickly identify spillovers into the three most important agricultural species worldwide.