The plant protein NbP3IP directs degradation ofRice stripe virusp3 silencing suppressor protein to limit virus infection through interaction with the autophagy-related protein NbATG8
The plant protein NbP3IP directs degradation ofRice stripe virusp3 silencing suppressor protein to limit virus infection through interaction with the autophagy-related protein NbATG8
复制标题
植物蛋白NbP3IP通过与自噬相关蛋白NbATG8相互作用，指导水稻条纹病毒p3沉默抑制蛋白的降解，从而限制病毒感染
DOI：
10.1111/nph.16917
复制
发表时间：
2020-10-04
期刊：
影响因子：
9.4
通讯作者：
Yan, Fei
中科院分区：
文献类型：
--
作者：
Jiang, Liangliang;Lu, Yuwen;Yan, Fei
在植物中，自噬参与对病毒感染的反应。然而，宿主因子在介导自噬以抑制病毒中的作用知之甚少。一种以前未鉴定的植物蛋白NbP 3 IP被证明与水稻条纹病毒（RSV）编码的RNA沉默抑制蛋白p3相互作用。研究了NbP 3 IP在RSV感染中的潜在作用。NbP 3 IP通过自噬途径降解p3，从而影响p3的沉默抑制活性。转NbP 3 IP基因本塞姆氏烟草对RSV感染的抗性RSV感染在ATG 5-或ATG 7-沉默的植物中被促进，并且在自噬被激活的GAPC-沉默的植物中被抑制，证实了自噬在抑制RSV感染中的作用。NbP 3 IP与NbATG 8 f相互作用，表明P3 IP具有潜在的选择性自噬体货物受体作用。此外，水稻NbP 3 IP同源物（OsP 3 IP）也介导p3降解并与OsATG 8b和p3相互作用。通过鉴定P3 IP参与自噬介导的RSV p3降解，我们揭示了一种新的抗RSV感染的机制，从而首次证明自噬可以发挥抗负链RNA病毒的作用。
In plants, autophagy is involved in responses to viral infection. However, the role of host factors in mediating autophagy to suppress viruses is poorly understood. A previously uncharacterized plant protein, NbP3IP, was shown to interact with p3, an RNA-silencing suppressor protein encoded byRice stripe virus(RSV), a negative-strand RNA virus. The potential roles of NbP3IP in RSV infection were examined. NbP3IP degraded p3 through the autophagy pathway, thereby affecting the silencing suppression activity of p3. Transgenic overexpression of NbP3IP conferred resistance to RSV infection inNicotiana benthamiana. RSV infection was promoted in ATG5- or ATG7-silenced plants and was inhibited in GAPC-silenced plants where autophagy was activated, confirming the role of autophagy in suppressing RSV infection. NbP3IP interacted with NbATG8f, indicating a potential selective autophagosomal cargo receptor role for P3IP. Additionally, the rice NbP3IP homolog (OsP3IP) also mediated p3 degradation and interacted with OsATG8b and p3. Through identification of the involvement of P3IP in the autophagy-mediated degradation of RSV p3, we reveal a new mechanism to antagonize the infection of RSV, and thereby provide the first evidence that autophagy can play an antiviral role against negative-strand RNA viruses.