Bacteriorhodopsin/amphipol complexes: Structural and functional properties
Bacteriorhodopsin/amphipol complexes: Structural and functional properties
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DOI：
10.1529/biophysj.107.121848
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发表时间：
2008-05-01
影响因子：
3.4
通讯作者：
Ebel, Christine
中科院分区：
文献类型：
--
作者：
Gohon, Yann;Dahmane, Tassadite;Ebel, Christine
两亲性聚合物Amphipol（APol）A8-35可使细菌视紫红质（BR）在无去污剂的情况下保持其天然可溶性数月。在一次光化之后。灰分，A8-35-络合的BR经历一个完整的光循环，动力学介于洗涤剂溶液和其天然膜之间。BR/APol复合物形成明确定义的球形颗粒，其包含BR单体、一套完整的紫色膜脂质，并且在外围分布中，类似于2 g APol/g BR，排列在致密层中。在不存在游离APol的情况下，BR/APol颗粒可以自缔合成小或大的有序原纤维。
The membrane protein bacteriorhodopsin ( BR) can be kept soluble in its native state for months in the absence of detergent by amphipol (APol) A8-35, an amphiphilic polymer. After an actinic. ash, A8-35-complexed BR undergoes a complete photocycle, with kinetics intermediate between that in detergent solution and that in its native membrane. BR/APol complexes form well defined, globular particles comprising a monomer of BR, a complete set of purple membrane lipids, and, in a peripheral distribution, similar to 2 g APol/g BR, arranged in a compact layer. In the absence of free APol, BR/APol particles can autoassociate into small or large ordered fibrils.