ROLE OF ANAEROBIC-BACTERIA IN THE METABOLIC WELFARE OF THE COLONIC MUCOSA IN MAN
ROLE OF ANAEROBIC-BACTERIA IN THE METABOLIC WELFARE OF THE COLONIC MUCOSA IN MAN
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DOI：
10.1136/gut.21.9.793
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发表时间：
1980-01-01
期刊：
影响因子：
24.5
通讯作者：
ROEDIGER, WEW
中科院分区：
文献类型：
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作者：
ROEDIGER, WEW
来自人结肠的分离的上皮细胞（结肠细胞）的悬浮液用于评估体内结肠粘膜通常可获得的呼吸燃料的利用。从升结肠（7）和降结肠（7）的手术标本制备细胞。使用的燃料是短链脂肪酸正丁酸酯，仅由结肠腔中的厌氧细菌产生，以及通常存在于循环中的葡萄糖和谷氨酰胺。当正丁酸是唯一底物时，升结肠中的O2消耗百分比为73%，降结肠中为75%。在10 mM葡萄糖存在下，这些比例变为59%和72%。在升结肠和降结肠中都观察到有氧糖酵解。葡萄糖氧化占升结肠耗氧量的85%，降结肠耗氧量的30%。在10 mM正丁酸盐的存在下，这些比例在升结肠中降低至41%，在降结肠中降低至16%。基于分离的结肠细胞中的事件反映体内燃料利用的假设，假设厌氧菌的脂肪酸是结肠粘膜，特别是远端结肠的主要能量来源。
Suspensions of isolated epithelial cells (colonocytes) from the human colon were used to assess utilization of respiratory fuels which are normally available to the colonic mucosa in vivo. Cells were prepared from operative specimens of the ascending colon (7) and descending colon (7). The fuels used were the short chain fatty acid n-butyrate, produced only by anerobic bacteria in the colonic lumen, together with glucose and glutamine, normally present in the circulation. The percentage O2 consumption attributable to n-butyrate, when this was the only substrate, was 73% in the ascending colon and 75% in the descending colon. In the presnce of 10 mM glucose these proportions changed to 59% and 72%. Aerobic glycolysis was observed in both the ascending and descending colon. Glucose oxidation accounted for 85% of the O2 consumption in the ascending colon and 30% in the descending colon. In the presence of 10 mM n-butyrate these proportions decreased to 41% in the ascending colon and 16% in the descending colon. Based on the assumption that events in the isolated colonocytes reflect utilization of fuels in vivo, the hypothesis that fatty acids of anaerobic bacteria are a major source of energy for the colonic mucosa, particularly of the distal colon.