Kinetics of the conversion of leukotriene C by γ-glutamyl transpeptidase
Kinetics of the conversion of leukotriene C by γ-glutamyl transpeptidase
复制标题
γ-谷氨酰转肽酶转化白三烯 C 的动力学
DOI：
10.1016/0006-291x(82)91255-4
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发表时间：
1982
影响因子：
3.1
通讯作者：
S. Hammarström
中科院分区：
文献类型：
--
作者：
L. Örning;S. Hammarström
γ-谷氨酰转肽酶(EC 2.3.2.2)通过去除谷氨基残基将白三烯C转化为白三烯D。在相同条件下，白三烯C4的米氏常数为5.6μM，还原型谷胱甘肽的Km值为5.7μM。这表明在生理条件下，白三烯C4和谷胱甘肽可能是γ-谷氨酰转肽酶的竞争底物。等摩尔量的L丝氨酸和硼酸钠抑制白三烯C4水解的表观KI为0.8 mM。
γ-Glutamyl transpeptidase (EC 2.3.2.2) converts leukotriene C to leukotriene D by removal of a glutamyl residue. The Michaelis constant for leukotriene C4hydrolysis was found to be 5.6 μM. Under the same conditions the Kmvalue for hydrolysis of reduced glutathione was 5.7 μM. This suggests that leukotriene C4and glutathione may be competing substrates for γ-glutamyl transpeptidase under physiological conditions. The apparent KIfor inhibition of leukotriene C4hydrolysis by equimolar amounts of L-serine and sodium borate was 0.8 mM.