STRESS PREFERENTIALLY INCREASES EXTRANEURONAL LEVELS OF EXCITATORY AMINO-ACIDS IN THE PREFRONTAL CORTEX - COMPARISON TO HIPPOCAMPUS AND BASAL GANGLIA
STRESS PREFERENTIALLY INCREASES EXTRANEURONAL LEVELS OF EXCITATORY AMINO-ACIDS IN THE PREFRONTAL CORTEX - COMPARISON TO HIPPOCAMPUS AND BASAL GANGLIA
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DOI：
10.1111/j.1471-4159.1993.tb13387.x
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发表时间：
1993-05-01
影响因子：
4.7
通讯作者：
MOGHADDAM, B
中科院分区：
文献类型：
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作者：
MOGHADDAM, B
采用脑内微透析技术研究了应激对大鼠内侧前额叶皮层、海马、纹状体和延髓核细胞外兴奋性氨基酸谷氨酸和天冬氨酸浓度的影响。一个20分钟的限制程序导致细胞外谷氨酸在所有地区的测试增加。前额叶皮层谷氨酸水平的增加明显高于其他区域。除纹状体外，所有区域的细胞外天冬氨酸水平均升高。此外，前额叶皮层中天冬氨酸水平的增加显著高于海马和丘脑核。局部灌注河豚毒素在限制程序显着降低了应激诱导的细胞外兴奋性氨基酸的增加。为了确保上述结果不是与压力无关的约束伪影（例如，降低的运动性），我们还检测了游泳应激对选定区域中兴奋性氨基酸的细胞外水平的影响，即，纹状体和内侧前额叶皮质。这两个区域显示出在室温水中游泳20分钟后，细胞外的天冬氨酸和谷氨酸水平显着增加。这项研究提供了直接的证据表明，压力增加神经元释放兴奋性氨基酸的区域选择性的方式。本研究结果的影响，应激诱导的儿茶酚胺释放和/或海马变性进行了讨论。
The technique of intracerebral microdialysis was used to assess the effect of stress on the extracellular concentrations of excitatory amino acids, glutamate and aspartate, in the rat medial prefrontal cortex, hippocampus, striatum, and nucleus accumbens. A 20-min restraint procedure led to an increase in extracellular glutamate in all regions tested. The increase in glutamate levels was significantly higher in the prefrontal cortex than that observed in other regions. With the exception of the striatum, extracellular levels of aspartate were increased in all regions. Furthermore, the increase in aspartate levels was significantly higher in prefrontal cortex compared to hippocampus and nucleus accumbens. Local perfusion of tetrodotoxin during the restraint procedure significantly decreased the stress-induced increase in extracellular excitatory amino acids. In order to ensure that the above results were not an artifact of restraint not associated with stress (e.g., decreased mobility), we also examined the effect of swimming stress on the extracellular levels of excitatory amino acids in selected regions, i.e., striatum and medial prefrontal cortex. Both regions displayed a significant increase in extracellular levels of aspartate and glutamate following 20 min of swimming in room temperature water. This study provides direct evidence that stress increases the neuronal release of excitatory amino acids in a regionally selective manner. The implications of the present findings for stress-induced catecholamine release and/or hippocampal degeneration are discussed.