5-Hydroxytryptamine-induced bladder hyperactivity via the 5-HT2A receptor in partial bladder outlet obstruction in rats.
5-Hydroxytryptamine-induced bladder hyperactivity via the 5-HT2A receptor in partial bladder outlet obstruction in rats.
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5-羟色胺通过 5-HT2A 受体诱导部分膀胱出口梗阻的大鼠膀胱过度活跃。
DOI：
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
H. Kuriyama
中科院分区：
文献类型：
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作者：
T. Sakai;Ken;Ken;I. Ikegaki;H. Kuriyama
我们研究了部分膀胱出口梗阻（BOO）对大鼠膀胱5-羟色胺（5-HT）受体亚型功能和基因表达的影响。观察假手术对照组和BOO大鼠离体膀胱的等距收缩。BOO大鼠膀胱条对5-HT的收缩反应显著增加，而对KCl、氯乙醇或苯肾上腺素的反应与对照组没有差异。5-HT（2A）受体拮抗剂酮色林可抑制BOO大鼠膀胱条5-HT诱导的过度收缩。膀胱条对5-羟色胺的收缩反应不受从完整膀胱中取出尿路上皮的影响。采用RT-PCR方法分析5-HT受体亚型基因在膀胱中的表达。对照组和BOO大鼠膀胱均检测到5-HT（2A）、5-HT（2B）、5-HT（2C）、5-HT(4)和5-HT(7)受体的mRNA表达。定量RT-PCR分析显示，BOO大鼠膀胱中5-HT（2A）受体mRNA较对照组明显升高。另一方面，BOO大鼠膀胱中5-HT(4)受体的基因表达未发生变化。这些结果表明，BOO大鼠膀胱对5-HT的收缩反应增加可能部分是由逼尿肌平滑肌5-HT（2A）受体上调引起的。
We investigated the effects of partial bladder outlet obstruction (BOO) on the function and gene expression of 5-hydroxytryptamine (5-HT) receptor subtypes in rat bladder. Isometric contractions of the isolated bladders from sham-operated control and BOO rats were examined. The contractile responses to 5-HT were significantly increased in BOO rat bladder strips, while the responses to KCl, carbachol, or phenylephrine were not different from the control. The 5-HT-induced hypercontraction in BOO rat bladder strips was inhibited by ketanserin, a 5-HT(2A) receptor antagonist. The contractile responses to 5-HT in bladder strips were not affected by urothelium removal from the intact bladder. The gene expression of 5-HT receptor subtypes in the bladders was analyzed by RT-PCR. The mRNA expression of the 5-HT(2A), 5-HT(2B), 5-HT(2C), 5-HT(4), and 5-HT(7) receptors was detected in both the control and BOO rat bladders. Quantitative RT-PCR analysis showed there was a significant increase of 5-HT(2A) receptor mRNA in the BOO rat bladder compared with the control bladder. On the other hand, the gene expression of the 5-HT(4) receptor was not changed in the BOO rat bladder. These results suggest that the increased contractile responses to 5-HT in BOO rat bladder may be partly caused by 5-HT(2A) receptor upregulation in the detrusor smooth muscles.
DOI：
10.1016/s0021-9258(19)39437-2
发表时间：
1990-04
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Paul R. Albert;Qun-Yong Zhou;H. V. Tol;J. Bunzow;O. Civelli
通讯作者：
Paul R. Albert;Qun-Yong Zhou;H. V. Tol;J. Bunzow;O. Civelli