Neprilysin II: A putative novel metalloprotease and its isoforms in CNS and testis.
Neprilysin II: A putative novel metalloprotease and its isoforms in CNS and testis.
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脑啡肽酶 II：一种假定的新型金属蛋白酶及其中枢神经系统和睾丸中的亚型。
DOI：
10.1006/bbrc.2000.2664
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发表时间：
2000
影响因子：
3.1
通讯作者：
Jean
中科院分区：
文献类型：
--
作者：
Ouimet Tanja;Patricia Facchinetti;C. Rose;Marie;Claude Gros;Jean
M13亚家族的金属蛋白酶，即包括奈普赖辛（NEP）和内皮素转化酶（ECE），参与各种神经元肽和激素肽的代谢，其抑制剂已经导致治疗上有用的药剂。利用同源克隆技术，我们在大鼠组织中鉴定了该家族的一个新成员。它是一种774个氨基酸的糖基化II型膜整合蛋白，含有锌结合共有基序，与NEP高度同源，因此被命名为NEPII。我们的特点是多个剪接变异体的NEPII mRNA不同的表达模式，在脑区，垂体和睾丸。睾丸的原位杂交，NEPII基因转录水平是最高的，揭示了一个定位在圆形精子细胞。在脑中，NEPII在几个神经元群体中不均匀地表达，并且根据与NEP的模式大致互补的模式。
Metalloproteases of the M13 subfamily, comprising namely neprylisin (NEP) and endothelin-converting enzyme (ECE), are involved in the metabolism of various neuronal and hormonal peptides, and inhibitors thereof have already led to therapeutically useful agents. Using homology cloning, we have identified a new member of this family in rat tissues. It is a glycosylated, type II integral membrane protein of 774 amino acids, containing a zinc-binding consensus motif, highly homologous to NEP and, therefore, designated NEPII. We have characterized multiple splice variants of NEPII mRNA with distinct expression patterns in brain regions, pituitary and testis. In situ hybridization of testis, where levels of the NEPII gene transcript are the highest, reveals a localization within round spermatids. In brain, NEPII is expressed heterogeneously among several neuronal populations and according to a pattern grossly complementary to that of NEP.