Pre-procedural expression of Mac-1 and LFA-1 on leukocytes for prediction of late restenosis and their possible correlation with advanced coronary artery disease
Pre-procedural expression of Mac-1 and LFA-1 on leukocytes for prediction of late restenosis and their possible correlation with advanced coronary artery disease
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DOI：
10.1002/cyto.b.10036
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发表时间：
2003-05-01
影响因子：
3.4
通讯作者：
Tárnok, A
中科院分区：
文献类型：
--
作者：
Rahimi, K;Maerz, HK;Tárnok, A
背景：炎症系统的激活状态被认为在预测再狭窄中起重要作用。白细胞粘附分子的激活发生在冠状动脉介入治疗后，并且激活水平与再狭窄相关。然而，很少有人知道干预前粘附分子的具体作用。本研究的目的是寻找所选粘附分子的表达水平的差异，以确定合适的工具，再狭窄患者的前程序识别前angioplasty.Methods：血液样本的31例择期冠状动脉造影术前获得的干预。还招募了7名健康志愿者。通过流式细胞术评估白细胞粘附分子Mac-1（CD 11b/CD 18）、LFA-1（CD 11 a/CD 18）、L-选择素（CD 62 L）、ICAM-1（CD 54）和MHC-II（HLA-DR）的表面表达。结果：根据临床和冠状动脉造影资料，将患者分为对照组（14例）、无再狭窄组（11例）、再狭窄组（4例）和晚期冠状动脉病变组（9例）。再狭窄组和晚期CAD组单核细胞和中性粒细胞Mac-1和LFA-1的表达高于其他组。使用术前表达水平，再狭窄患者可以通过与CD 11 a，CD 11b和CD 18（平均识别指数= 95.5%）的判别分析进行预测。结论：该初步研究的数据表明，术前CD 11 a和CD 11b的活化状态可能在随后的再狭窄的发展中发挥作用。此外，CD 11 a、CD 11b和CD 18可能有助于作为CAD进展的指标。(C)2003 Wiley-Liss，Inc.
Background: The activation status of the inflammatory system has been suggested to play an important role in predicting restenosis. Activation of leukocyte adhesion molecules occur after coronary intervention and the level of activation correlates to restenosis. However, little is known about the specific role of adhesion molecules before intervention. The purpose of this study concerned the search for differences in the expression level of selected adhesion molecules to identify suitable tools for the pre-procedural identification of restenosis patients prior to angioplasty.Methods: Blood samples of 31 patients undergoing elective coronary angiography were obtained just before intervention. Seven healthy volunteers were also enrolled. Surface expression of leukocyte adhesion molecules Mac-1 (CD11b/CD18), LFA-1 (CD11a/CD18), L-Selectin (CD62L), ICAM-1 (CD54), and MHC-II (HLA-DR) were assessed by flow cytometry. Patients with a successful angioplasty received a follow-up angiography after six months.Results: According to the clinical and angiographic data, patients were divided into four groups: control (N = 14), no restenosis (N = 11), restenosis (N = 4), and advanced coronary artery disease (CAD, N = 9). The restenosis group and the advanced CAD group showed higher expression of Mac-1 and LFA-1 on monocytes and neutrophils compared to the other groups. Using the pre-procedural expression levels, patients with restenosis could be predicted by discriminant analysis with CD11a, CD11b, and CD18 (average recognition index = 95.5%).Conclusions: The data of this pilot study indicate that pre-procedural activation status of CD11a and CD11b may play a role in the subsequent development of restenosis. Moreover, CD11a, CD11b, and CD18 may be helpful as indicators for the progression of CAD. (C) 2003 Wiley-Liss, Inc.