Fabrication of three-dimensional calcium alginate hydrogels using sacrificial templates of sugar
Fabrication of three-dimensional calcium alginate hydrogels using sacrificial templates of sugar
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DOI：
10.1016/j.jbiosc.2020.06.014
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发表时间：
2020-11-01
影响因子：
2.8
通讯作者：
Shiku, Hitoshi
中科院分区：
文献类型：
--
作者：
Ino, Kosuke;Fukuda, Mika T.;Shiku, Hitoshi
水凝胶在生物应用方面受到越来越多的关注。在水凝胶中，海藻酸钙（Ca-alginate）水凝胶具有生物相容性好、低毒性、成本低等优点，并且可以通过Ca2+和海藻酸钠（Na-alginate）的简单混合快速制备而得到广泛应用。为了将水凝胶应用于生物领域，有必要构建设计好的水凝胶结构。虽然已经提出了几种方法来制造设计的水凝胶，但一种简单和低成本的方法是可取的。因此，我们开发了一种新的方法，利用糖结构的牺牲模板来制造三维（3D）设计的海藻酸钙水凝胶。在这种方法中，将海藻酸钠溶液与熔融糖混合，并将所得的高粘性材料用作3D糖结构的模具作为牺牲模板。由于糖结构与水凝胶相比易于处理，因此糖模板对于制备3D结构非常有用。最后，将糖和海藻酸钠结构浸入CaCl2溶液中，使模板同时溶解，形成海藻酸钙水凝胶。得到的水凝胶具有糖模板的形状。通过堆叠和融合各种糖结构，如纤维和块，3D设计的海藻酸钙水凝胶可以成功制造。这种简单、低成本的方法在各种生物应用中显示出良好的应用潜力。(C) 2020，生物技术学会，日本。版权所有。
Hydrogels are receiving increasing attention in bioapplications. Among hydrogels, calcium alginate (Ca-alginate) hydrogels are widely used for their biocompatibility, low toxicity, low cost, and rapid fabrication by simple mixing of Ca2+ and sodium alginate (Na-alginate). For bioapplications using hydrogels, it is necessary to construct designed hydrogel structures. Although several methods have been proposed for fabricating designed hydrogels, a simple and low-cost method is desirable. Therefore, we developed a new method using sacrificial templates of sugar structures to fabricate three-dimensional (3D) designed Ca-alginate hydrogels. In this method, Na-alginate solution is mixed with molten sugar, and the resulting highly viscous material used to mold 3D sugar structures as sacrificial templates. Since sugar constructs are easily handled compared to hydrogels, sugar templates are useful for preparing 3D constructs. Finally, the sugar and Na-alginate structure is immersed in a CaCl2 solution to simultaneously dissolve the template and form the Ca-alginate hydrogel. The resulting hydrogel takes the shape of the sugar template. By stacking and fusing various sugar structures, such as fibers and blocks, 3D designed Ca-alginate hydrogels can be successfully fabricated. This simple and low-cost method shows excellent potential for application to a variety of bioapplications. (C) 2020, The Society for Biotechnology, Japan. All rights reserved.