Characterization of an isolate of pepper mild mottle tobamovirus occurring in Canada
Characterization of an isolate of pepper mild mottle tobamovirus occurring in Canada
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DOI：
10.1080/07060669709500579
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发表时间：
1997-03-01
影响因子：
2
通讯作者：
Hamilton, RI
中科院分区：
文献类型：
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作者：
Beczner, L;Rochon, DM;Hamilton, RI
从里士满的商业辣椒生产领域中的抗烟草花叶病毒（TMV）的匈牙利甜椒（Capsicum annuum）品种中分离到一种花叶病毒，并且根据实验宿主范围、血清学、形态学和cDNA-RNA杂交，证明该病毒是辣椒温和斑驳病毒（PMMV）的菌株。PMMV的模式株和非常相似的TMV的Samsun潜伏株（SLV）似乎比加拿大分离物在基因组上更相似。从里士满和奥利弗，不列颠哥伦比亚省的种植者提供的种子缓冲液中分离出几种PMMV，但PMMV仅从里士满样品中分离出，这表明该疾病在加拿大的发生是种植受感染或污染的种子的结果。
A tobamovirus was isolated from tobacco mosaic virus (TMV)-resistant Hungarian cultivars of sweet pepper (Capsicum annuum) in commercial pepper production fields at Richmond, BC, and shown to be a strain of pepper mild mottle virus (PMMV) on the basis of experimental host range, serology, morphology, and cDNA-RNA hybridization. The type strain of PMMV and the very similar Samsun latent strain of TMV (SLV) appeared to be more similar to each other genomically than to the Canadian isolate. Several tobamoviruses were isolated from buffer-washes of seed furnished by growers in Richmond and Oliver, BC, but PMMV was isolated only from Richmond samples, suggesting that the occurrence of the disease in Canada was a consequence of planting infected or contaminated seed.