Pervasive conformational fluctuations on microsecond time scales in a fibronectin type III domain
Pervasive conformational fluctuations on microsecond time scales in a fibronectin type III domain
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DOI：
10.1038/nsb0198-55
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发表时间：
1998-01-01
期刊：
影响因子：
--
通讯作者：
Palmer, AG
中科院分区：
文献类型：
--
作者：
Akke, M;Liu, J;Palmer, AG
利用一种新颖的非共振旋转框架N-15核磁共振自旋弛豫实验，表征了人tenascin-C第三型纤维连接蛋白III型结构域32 ~ 175 μ s的构象波动。III型结构域β -夹层结构的连续区域的构象波动可以表示集体运动，例如β -薄片的瞬态扭曲或呼吸。含有Arg-Gly-Asp （RGD）三肽的环的柔韧性可能影响该基序在蛋白质-蛋白质相互作用中的可及性。
A novel off-resonance rotating-frame N-15 NMR spin relaxation experiment is used to characterize conformational fluctuations with correlation times between 32 and 175 mu s in the third fibronectin type III domain of human tenascin-C. Conformational fluctuations of contiguous regions of the beta-sandwich structure of the type III domain may represent collective motions, such as transient twisting or breathing of the beta-sheets. Flexibility of the loop containing the Arg-Gly-Asp (RGD) tripeptide may affect the accessibility of this motif in protein-protein interactions.