Sex differences in skin carotenoid deposition and acute UVB-induced skin damage in SKH-1 hairless mice after consumption of tangerine tomatoes.
Sex differences in skin carotenoid deposition and acute UVB-induced skin damage in SKH-1 hairless mice after consumption of tangerine tomatoes.
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食用橘子西红柿后 SKH-1 无毛小鼠皮肤类胡萝卜素沉积和急性 UVB 引起的皮肤损伤的性别差异。
DOI：
10.1002/mnfr.201500317
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发表时间：
2015
影响因子：
5.2
通讯作者：
Oberyszyn,TatianaM
中科院分区：
文献类型：
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作者：
Kopec,RachelE;Schick,Jonathan;Tober,KathleenL;Riedl,KenM;Francis,DavidM;Young,GregoryS;Schwartz,StevenJ;Oberyszyn,TatianaM
紫外线暴露是皮肤癌发生的一个主要因素，其性别效应不同。食用番茄制品可降低人体UVB诱导的红斑强度，但其机制尚不清楚。方法和结果将4周龄SKH‐1无毛小鼠（雌雄各40只）分为两组（对照组或含有10%富含紫外线吸收植物烯和植物流感素的柑橘番茄）和两组（有或无紫外线）。饲养10周后，紫外线组单次照射，48 h后处死。采集血液和背部皮肤样本进行类胡萝卜素分析。采集背部皮肤，评估性别和紫外线对类胡萝卜素沉积、炎症（皮褶厚度、髓过氧化物酶水平）和DNA损伤（环丁烷嘧啶二聚体、p53）的影响。女性皮肤和血液中的类胡萝卜素含量明显高于男性。紫外线暴露显著降低了女性皮肤类胡萝卜素水平，但男性没有。在两性中，番茄的食用降低了急性紫外线诱导的CPD的增加，并降低了雄性中髓过氧化物酶活性和p53阳性表皮细胞的百分比。结论橘皮番茄通过降低雄性小鼠的DNA损伤和减少炎症，介导了紫外线诱导的SKH - 1小鼠急性皮肤损伤。
ScopeUVB exposure, a major factor in the development of skin cancer, has differential sex effects. Tomato product consumption reduces the intensity of UVB‐induced erythema in humans, but the mechanisms are unknown.Methods and resultsFour‐week‐old SKH‐1 hairless mice (40 females, 40 males) were divided into two feeding groups (control or with 10%tangerinetomatoes naturally rich in UV‐absorbing phytoene and phytofluene) and two UV exposure groups (with or without UV). After 10 weeks of feeding, the UV group was exposed to a single UV dose and sacrificed 48 h later. Blood and dorsal skin samples were taken for carotenoid analysis. Dorsal skin was harvested to assess sex and UV effects on carotenoid deposition, inflammation (skinfold thickness, myeloperoxidase levels), and DNA damage (cyclobutane pyrimidine dimers, p53). Females had significantly higher levels of both skin and blood carotenoids relative to males. UV exposure significantly reduced skin carotenoid levels in females but not males. Tomato consumption attenuated acute UV‐induced increases in CPD in both sexes, and reduced myeloperoxidase activity and percent p53 positive epidermal cells in males.ConclusionTangerinetomatoes mediate acute UV‐induced skin damage in SKH‐1 mice via reduced DNA damage in both sexes, and through reduced inflammation in males.