Activation of leukemia viruses by graft-versus-host and mixed lymphocyte reactions in vitro.
Activation of leukemia viruses by graft-versus-host and mixed lymphocyte reactions in vitro.
复制标题
体外移植物抗宿主反应和混合淋巴细胞反应激活白血病病毒。
DOI：
--
复制
发表时间：
1972
影响因子：
11.1
通讯作者：
C. Carpenter
中科院分区：
文献类型：
--
作者：
M. Hirsch;Phillips Sm;C. Solnik;P. Black;R. Schwartz;C. Carpenter
BALB/c或CAF（1）小鼠的脾细胞在体外培养2-7天时释放很少或检测不到白血病病毒。与此相反，CAF（1）小鼠的脾细胞预先接种亲本BALB/c脾细胞释放白血病病毒在10/11例研究。正常BALB/c和CAF（1）小鼠脾细胞的混合物培养物也含有白血病病毒。在CAF（1）或BALB/c脾细胞培养物中，植物血凝素诱导淋巴细胞转化，但这种转化不激活白血病病毒。它的结论是，混合淋巴细胞培养物在体外，就像移植物抗宿主反应在体内，可以激活白血病病毒，通常存在于一个压抑的形式。这种激活不仅仅是淋巴细胞转化的功能。活化的小鼠白血病病毒可能随后解释了移植物抗宿主病中观察到的高肿瘤发生率。
Spleen cells from BALB/c or CAF(1) mice released little or no detectable leukemia virus when cultured 2-7 days in vitro. In contrast, spleen cells of CAF(1) mice previously inoculated with parental BALB/c spleen cells released leukemia viruses in 10 of 11 cases studied. Cultures of a mixture of spleen cells from normal BALB/c and CAF(1) mice also contained leukemia viruses. Phytohemagglutinin induced the transformation of lymphocytes in cultures of CAF(1) or BALB/c spleen cells, but this transformation did not activate leukemia viruses. It is concluded that mixed lymphocyte cultures in vitro, just as graft-versus-host reactions in vivo, can activate leukemia viruses that are normally present in a repressed form. This activation is not solely a function of lymphocyte transformation. The activated mouse leukemia virus may subsequently account for the observed high incidence of neoplasia in graft-versus-host disease.