Novel system using microliter order sample volume for measuring arterial radioactivity concentrations in whole blood and plasma for mouse PET dynamic study
Novel system using microliter order sample volume for measuring arterial radioactivity concentrations in whole blood and plasma for mouse PET dynamic study
复制标题
DOI：
10.1088/0031-9155/58/22/7889
复制
发表时间：
2013-11-21
影响因子：
3.5
通讯作者：
Kanno, Iwao
中科院分区：
文献类型：
--
作者：
Kimura, Yuichi;Seki, Chie;Kanno, Iwao
本研究旨在开发一种新的用于小鼠PET动力学研究的CD-Well系统。CD-Well允许测定动脉全血和血浆的时间-活性曲线（tac），每个样品使用2-3 μ L的血液；微小的样本量对于小动物的研究是理想的。该系统具有以下优点：(1)分别测量全血和血浆的体积和放射性；(2)允许以10秒为间隔进行测量，以捕捉TAC的初始快速变化；(3)结构紧凑，易于操作，最大限度地减少采样造成的失血，以及TAC的延迟和分散。CD-Well有36个u形通道。一滴血被取样到通道的开口并储存在那里。连续取样完成后，将CD-Well离心并使用平板扫描仪进行扫描，以确定血浆和血细胞的区域。测量的长度被转换成体积，因为通道具有精确和均匀的横截面。然后，将CD-Well暴露在成像板上以测量放射性。最后，计算放射性浓度。我们评估了CD-Well在体外测量和体内f -18-氟脱氧葡萄糖和[C-11] 2-碳甲氧基-3 β -（4-氟苯基）tropane研究中的性能。在体外评估中，在小鼠动态研究中测量的典型放射性范围内，与人工测量的百分比差异（平均+/- SE）为全血4.4 +/- 3.6%，血浆4.0 +/- 3.5%。在体内研究中，获得了合理的tac。峰被很好地捕获，时间过程与心室PET成像得到的TAC很好地吻合。总失血量小于200 μ L，对小鼠无生理影响。CD-Well具有良好的性能，可用于小鼠PET动力学研究。
This study aimed to develop a new system, named CD-Well, for mouse PET dynamic study. CD-Well allows the determination of time-activity curves (TACs) for arterial whole blood and plasma using 2-3 mu L of blood per sample; the minute sample size is ideal for studies in small animals. The system has the following merits: (1) measures volume and radioactivity of whole blood and plasma separately; (2) allows measurements at 10 s intervals to capture initial rapid changes in the TAC; and (3) is compact and easy to handle, minimizes blood loss from sampling, and delay and dispersion of the TAC. CD-Well has 36 U-shaped channels. A drop of blood is sampled into the opening of the channel and stored there. After serial sampling is completed, CD-Well is centrifuged and scanned using a flatbed scanner to define the regions of plasma and blood cells. The length measured is converted to volume because the channels have a precise and uniform cross section. Then, CD-Well is exposed to an imaging plate to measure radioactivity. Finally, radioactivity concentrations are computed. We evaluated the performance of CD-Well in in vitro measurement and in vivo F-18-fluorodeoxyglucose and [C-11] 2-carbomethoxy-3 beta-(4-fluorophenyl) tropane studies. In in vitro evaluation, per cent differences (mean +/- SE) from manual measurement were 4.4 +/- 3.6% for whole blood and 4.0 +/- 3.5% for plasma across the typical range of radioactivity measured in mouse dynamic study. In in vivo studies, reasonable TACs were obtained. The peaks were captured well, and the time courses coincided well with the TAC derived from PET imaging of the heart chamber. The total blood loss was less than 200 mu L, which had no physiological effect on the mice. CD-Well demonstrates satisfactory performance, and is useful for mouse PET dynamic study.