PARTIAL-PURIFICATION AND CHARACTERIZATION OF HEPATOCYTE GROWTH-FACTOR FROM SERUM OF HEPATECTOMIZED RATS
PARTIAL-PURIFICATION AND CHARACTERIZATION OF HEPATOCYTE GROWTH-FACTOR FROM SERUM OF HEPATECTOMIZED RATS
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DOI：
10.1016/0006-291x(84)91253-1
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发表时间：
1984-01-01
影响因子：
3.1
通讯作者：
ICHIHARA, A
中科院分区：
文献类型：
--
作者：
NAKAMURA, T;NAWA, K;ICHIHARA, A
当大鼠血清进行凝胶过滤Sephadex G-200柱上，一个因素，促肝细胞生长素，促进肝细胞生长的原代培养分离。它的分子量约为150 KD [千道尔顿]，是一种阴离子蛋白质，在酸和热处理时不稳定。通过肝素-琼脂糖CL-6 B亲和层析进一步纯化20倍的促肝细胞激素。纯化的促肝素在20 μ g/ml时有效，在120 μ g/ml时最有效，其作用与胰岛素加表皮生长因子的作用相加。在大鼠部分肝切除术后，血清中的促肝激素活性随时间增加，达到最大值约5倍的初始水平，手术后24小时。各种已知的生长因子，如成纤维细胞生长因子，血小板衍生生长因子，生长调节素，凝血酶和转铁蛋白，不刺激培养的肝细胞中的DNA合成。促肝细胞激素显然是一种新的生长因子。[肝细胞生长因子在肝再生中的作用的影响。]
When rat serum was subjected to gel filtration on a Sephadex G-200 column, a factor, hepatotropin, that promoted hepatocyte growth in primary culture was separated. Its MW was about 150 KD [kilodalton] and it was an anionic protein that was unstable on acid- and heat-treatments. Hepatotropin was purified 20-fold further by affinity chromatography on heparin-Sepharose CL-6B. The purified hepatotropin was effective at 20 .mu.g/ml and maximally effective at 120 .mu.g/ml and its effect was additive with that of insulin plus epidermal growth factor. In rats after partial hepatectomy, the hepatotropin activity in the serum increased time-dependently reaching a maximum of about 5-fold the initial level 24 h after the operation. Various known growth factors, such as fibroblast growth factor, platelet derived growth factor, somatomedin, thrombin and transferrin, did not stimulate DNA synthesis in cultured hepatocytes. Hepatotropin evidently is a new growth factor. [Implications with respect to the role of hepatocyte growth factor in liver regeneration are presented.].