Stokes shift spectroscopy pilot study for cancerous and normal prostate tissues
Stokes shift spectroscopy pilot study for cancerous and normal prostate tissues
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DOI：
10.1364/ao.51.003642
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发表时间：
2012-06-01
期刊：
影响因子：
1.9
通讯作者：
Alfano, R. R.
中科院分区：
文献类型：
--
作者：
Ebenezar, J.;Pu, Yang;Alfano, R. R.
斯托克斯位移光谱 (S3) 是一种新兴的癌症检测方法。本文的目的是评估 S3 技术在正常和癌性前列腺组织的检测和表征方面的诊断潜力。从八名患者中每人采集一对癌性和正常前列腺组织样本。斯托克斯位移光谱通过同时扫描激发和发射波长同时保持它们之间的固定波长间隔 Delta lambda = 20 nm 来测量。该技术的显着特点是在 250 至 600 nm 的波长范围内观察到正常和癌性前列腺组织之间的高分辨率发射峰和显着光谱差异。癌性前列腺组织和正常前列腺组织的斯托克斯位移光谱显示在 300、345、440 和 510 nm 附近有明显的峰，分别归因于色氨酸、胶原、NADH 和黄素。为了量化正常和癌性前列腺组织之间的光谱差异，计算了两个光谱比。结果显示，比率参数 R-1 = I-297/I-345 和 R-2 = I-307/I-345 都是出色的诊断比率参数，在区分癌组织和正常组织方面具有 100% 的特异性和 100% 的敏感性。我们的结果表明，S3 是一种用于检测癌性前列腺组织的灵敏且特异的技术。 (C) 2012年美国光学学会
Stokes shift spectroscopy (S3) is an emerging approach toward cancer detection. The goal of this paper is to evaluate the diagnostic potential of the S3 technique for the detection and characterization of normal and cancerous prostate tissues. Pairs of cancerous and normal prostate tissue samples were taken from each of eight patients. Stokes shift spectra were measured by simultaneously scanning both the excitation and emission wavelengths while keeping a fixed wavelength interval Delta lambda = 20 nm between them. The salient features of this technique are the highly resolved emission peaks and significant spectral differences between the normal and cancerous prostate tissues, as observed in the wavelength region of 250 to 600 nm. The Stokes shift spectra of cancerous and normal prostate tissues revealed distinct peaks around 300, 345, 440, and 510 nm, which are attributed to tryptophan, collagen, NADH, and flavin, respectively. To quantify the spectral differences between the normal and cancerous prostate tissues, two spectral ratios were computed. The findings revealed that both ratio parameters R-1 = I-297/I-345 and R-2 = I-307/I-345 were excellent diagnostic ratio parameters giving 100% specificity and 100% sensitivity for distinguishing cancerous tissue from the normal tissue. Our results demonstrate that S3 is a sensitive and specific technique for detecting cancerous prostate tissue. (C) 2012 Optical Society of America