Plasma ubiquinol-10 is decreased in patients with hyperlipidaemia
Plasma ubiquinol-10 is decreased in patients with hyperlipidaemia
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DOI：
10.1016/s0021-9150(96)06021-2
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发表时间：
1997-02-28
期刊：
影响因子：
5.3
通讯作者：
Beisiegel, U
中科院分区：
文献类型：
--
作者：
Kontush, A;Reich, A;Beisiegel, U
泛醇-10是泛醌-10（辅酶Q（10））的还原形式，是存在于几乎所有人体组织中的有效亲脂性抗氧化剂。泛醇-10的异常氧化不稳定性意味着它可能代表氧化应激的敏感指标。进行本研究以评估人血浆中泛醇-10的水平可以区分健康受试者和预期在体内经受增加的氧化应激的患者的假设。使用一种新开发的方法，我们测量了38例有或无进一步并发症（如冠心病、高血压或肝病）的糖尿病患者和30例健康受试者的血浆泛醇-10。发现与对照受试者相比，从糖尿病患者获得的血浆样品的氧化性增加，表明患者在体内比对照受到更高的氧化应激。血浆泛醇-10，表示为总泛醇-10+泛醌-10的百分比或归一化为血浆脂质，在患者中低于对照组（分别为P = 0.001和0.008）。泛醇-10比例按年轻对照组>老年对照组>无并发症的尿毒症患者>有并发症的尿毒症患者的顺序降低（P = 0.003）。泛醇-10比例与血浆甘油三酯呈负相关。高血压患者中泛醌醇-10的比例较低。当研究人群分为吸烟者和非吸烟者时，发现吸烟者的血浆泛醇-10减少，与其是否表示为总泛醇-10+泛醌-10的百分比（P = 0.006）或标准化为血浆脂质（P = 0.009）无关。这些数据表明，人血浆中泛醇-10的水平可能代表体内氧化应激的敏感指标，特别是指示早期氧化损伤。测量血浆泛醇-10可以作为评估人类氧化应激的实用方法。(C)1997 Elsevier Science爱尔兰有限公司
Ubiquinol-10, the reduced form of ubiquinone-10 (coenzyme Q(10)), is a potent lipophilic antioxidant present in nearly all human tissues. The exceptional oxidative lability of ubiquinol-10 implies that it may represent a sensitive index of oxidative stress. The present study was undertaken to assess the hypothesis that the level of ubiquinol-10 in human plasma can discriminate between healthy subjects and patients who are expected to be subjected to an increased oxidative stress in vivo. Using a newly developed method, we measured plasma ubiquinol-10 in 38 hyperlipidaemic patients with and without further complications, such as coronary heart disease, hypertension: or liver disease, and in 30 healthy subjects. The oxidizability of plasma samples obtained from hyperlipidaemic patients was found to be increased in comparison with control subjects, suggesting that the patients were subjected to a higher oxidative stress in vivo than the controls. Plasma ubiquinol-10, expressed as a percentage of total ubiquinol-10 + ubiquinone-10 or normalized to plasma lipids, was lower in the patients than in controls (P = 0.001 and 0.008, respectively). The proportion of ubiquinol-10 decreased in the order young controls > aged controls > hyperlipidaemic patients without complications > hyperlipidaemic patients with complications (P = 0.003). A negative correlation was found between the proportion of ubiquinol-10 and plasma triglycerides. The hyperlipidaemic patients with hypertension had a lower proportion of ubiquinol-10 than subjects without. When the study population was divided into smokers and non-smokers, plasma ubiquinol-10 was found to be reduced amongst smokers, independently of whether it was expressed as a percentage of total ubiquinol-10 + ubiquinone-10 (P = 0.006) or normalized to plasma lipids (P = 0.009). These data suggest that the level of ubiquinol-10 in human plasma may represent a sensitive index of oxidative stress in vivo especially indicative of early oxidative damage. Measuring plasma ubiquinol-10 can be proposed as a practical approach to assess oxidative stress in humans. (C) 1997 Elsevier Science Ireland Ltd.