LIPOPROTEIN PIGMENTS - THEIR RELATIONSHIP TO AGING IN HUMAN NERVOUS-SYSTEM .2. MELANIN CONTENT OF PIGMENTED NERVE-CELLS
LIPOPROTEIN PIGMENTS - THEIR RELATIONSHIP TO AGING IN HUMAN NERVOUS-SYSTEM .2. MELANIN CONTENT OF PIGMENTED NERVE-CELLS
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DOI：
10.1093/brain/97.1.489
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发表时间：
1974-01-01
期刊：
影响因子：
14.5
通讯作者：
YATES, PO
中科院分区：
文献类型：
--
作者：
MANN, DMA;YATES, PO
材料与方法在尸检时从45例年龄范围为新生儿至91岁的患者的脑中获得人黑质和蓝斑块，所有患者在死亡时均无明显的神经系统疾病。无异常神经病理学发现。将组织块固定在10%中性福尔马林中;在5 μ l和16 μ l下切割处理的石蜡切片。在16岁的时候剪的。RNA用Azure B（Shea，1970）染色，黑色素用Schmorl染色（Golodetz和Unna，1909）。神经元黑色素含量估计为580 nm处的光透射百分比，580 nm是施莫尔反应的普鲁士蓝-黑色素复合物的最大吸收。100%背景光强度阅读通过邻近测量细胞的未染色细胞质区域设置。每种类型至少测量100个细胞。透射率的测量值直接记录在穿孔带上，以便计算机分析（Mann，1973）。
MATERIALS AND METHODS Blocks of human substantia nigra and locus caeruleus were obtained at post-mortem examination from the brains of 45 patients of age range newborn to 91 years, all of whom were, at death, free from apparent neurological illness. There were no abnormal neuropathological findings. Blocks were fixed in 10 per cent neutral formalin; processed and paraffin sections were cut at 5 and 16\i. Those cut at 16 y. were stained with Azure B (Shea, 1970) for RNA and Schmorl's stain (Golodetz and Unna, 1909) for melanin. Neuronal melanin content was estimated as the percentage transmission of light at 580 nm, the absorption maximum of the Prussian blue-melanin complex of the Schmorl reaction. The 100 per cent background light intensity reading was set through an area of unstained cytoplasm adjacent to the measured cells. A minimum of 100 cells of each type were measured. Measurements of percentage transmission were recorded directly on to punched tape for computer analysis (Mann, 1973).