Complex Intratissue Microbiota Forms Biofilms in Periodontal Lesions
Complex Intratissue Microbiota Forms Biofilms in Periodontal Lesions
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DOI：
10.1177/0022034517732754
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发表时间：
2018-02-01
影响因子：
7.6
通讯作者：
Choi, Y.
中科院分区：
文献类型：
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作者：
Baek, K.;Ji, S.;Choi, Y.
牙周炎是由龈下菌群失调引起的，这些菌群可能导致细菌侵入牙龈组织的增加。虽然与从健康到牙周炎的转变相关的群落结构的变化已经得到了很好的表征，但牙周病变的牙龈组织内存在的细菌的性质尚不清楚。为了表征牙周病变组织内的微生物群并将其与菌斑微生物群进行比较，从7名慢性牙周炎患者中获得牙龈组织和龈下菌斑。16S rRNA基因的测序分析显示，物种丰富度和多样性没有显着差异，这两组之间。然而，组织内社区的个体间变异性小于斑块社区。此外，与菌斑群落相比，组织内群落的特点是厚壁菌门的丰度降低，梭菌门和绿弯菌门的丰度增加。特别是，具核梭杆菌和牙龈卟啉单胞菌在组织内高度富集，占总细菌的15%至40%。此外，生物膜，如通过阿辛蓝染色和原子力显微镜观察到的，在结缔组织纤维的降解突出的组织内观察到。总之，非常复杂的细菌群落以生物膜的形式存在于牙周病变的牙龈组织内，这可能是持续感染的储存库。这一新的发现可能会促进对牙周炎治疗策略的新研究。
Periodontitis is caused by dysbiotic subgingival bacterial communities that may lead to increased bacterial invasion into gingival tissues. Although shifts in community structures associated with transition from health to periodontitis have been well characterized, the nature of bacteria present within the gingival tissue of periodontal lesions is not known. To characterize microbiota within tissues of periodontal lesions and compare them with plaque microbiota, gingival tissues and subgingival plaques were obtained from 7 patients with chronic periodontitis. A sequencing analysis of the 16S rRNA gene revealed that species richness and diversity were not significantly different between the 2 groups. However, intersubject variability of intratissue communities was smaller than that of plaque communities. In addition, when compared with the plaque communities, intratissue communities were characterized by decreased abundance of Firmicutes and increased abundance of Fusobacteria and Chloroflexi. In particular, Fusobacterium nucleatum and Porphyromonas gingivalis were highly enriched within the tissue, composing 15% to 40% of the total bacteria. Furthermore, biofilms, as visualized by alcian blue staining and atomic force microscopy, were observed within the tissue where the degradation of connective tissue fibers was prominent. In conclusion, very complex bacterial communities exist in the form of biofilms within the gingival tissue of periodontal lesions, which potentially serve as a reservoir for persistent infection. This novel finding may prompt new research on therapeutic strategies to treat periodontitis.