Cloning of minimally divergent allelic forms of the natural killer (NK) receptor Ly-49C, differentially controlled by host genes in the MHC and NK gene complexes.
Cloning of minimally divergent allelic forms of the natural killer (NK) receptor Ly-49C, differentially controlled by host genes in the MHC and NK gene complexes.
复制标题
自然杀伤 (NK) 受体 Ly-49C 的最小差异等位基因形式的克隆，由 MHC 和 NK 基因复合体中的宿主基因进行差异控制。
DOI：
10.4049/jimmunol.157.9.3936
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发表时间：
1996
影响因子：
4.4
通讯作者：
C. Sentman
中科院分区：
文献类型：
--
作者：
J. Sundbäck;K. Kärre;C. Sentman
我们研究了单抗SW5E6检测到的Ly-49C的表达是否与Ly-49A一样受到宿主MHC的调控。在A/Sn (A)和C57BL/6 (B)来源的小鼠中，Ly-49C的表达受宿主MHC的调节存在差异。对AXB重组自交系的分析表明，AXB有两个基因座调控其表达，一个与NK基因复合物分离，另一个与MHC分离。在MHC同源菌株中，Ly-49C的A型表达水平较低，当H-2b单倍型存在时，Ly-49C的A型表达水平进一步下调（极低）。B型Ly-49C高表达，在H-2Kk（中）存在下下调表达。为了验证A/Sn和C57BL/6小鼠的Ly-49C受体可能受到MHC产物的差异调节，因为它们代表等位基因形式，我们利用逆转录酶PCR从A/Sn和C57BL/6小鼠品系中克隆了Ly-49C的cdna。两个菌株的Ly-49C序列相差6个核苷酸，导致4个预测的氨基酸变化。这两种cdna在EL-4细胞中表达时均可被ly - 49c特异性抗体检测到。因此，与Ly-49A一样，Ly-49C的表达也受到MHC I类分子的调控。此外，A/Sn和C57BL/6小鼠表达不同形式的Ly-49C，这些Ly-49C被不同的MHC I类分子下调。
We have investigated whether expression of Ly-49C, detected by the mAb SW5E6, is subject to similar regulation by host MHC as is Ly-49A. Ly-49C expression was regulated by host MHC differentially in mice of A/Sn (A) and C57BL/6 (B) origin. Analysis of AXB recombinant inbred strains suggested two loci regulating the expression, one segregating with the NK gene complex and the other with the MHC. In MHC congenic strains, the A form of Ly-49C had a low expression level and was further down-regulated in the presence of the H-2b haplotype (very low). The B form of Ly-49C had a high expression level and was down-regulated in the presence of H-2Kk (medium). To test the hypothesis that the Ly-49C receptor in A/Sn and C57BL/6 mice may be differentially regulated by MHC products because they represent allelic forms, cDNAs for Ly-49C were cloned by reverse-transcriptase PCR from A/Sn and C57BL/6 mouse strains. The Ly-49C sequences from the two strains differed by six nucleotides leading to four predicted amino acid changes. Both cDNAs could be detected by the Ly-49C-specific Ab when expressed in EL-4 cells. Thus, like Ly-49A, Ly-49C expression is regulated by MHC class I molecules. Furthermore, A/Sn and C57BL/6 mice express different forms of Ly-49C that are down-regulated by different MHC class I molecules.