Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections Using Noninvasive Optical Imaging.
Preclinical Models and Methodologies for Monitoring Staphylococcus aureus Infections Using Noninvasive Optical Imaging.
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临床前模型和方法，用于使用非侵入性光学成像监测金黄色葡萄球菌感染。
DOI：
10.1007/978-1-4939-9849-4_15
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Miller LS
中科院分区：
文献类型：
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作者：
Archer NK;Wang Y;Ortines RV;Liu H;Nolan SJ;Liu Q;Alphonse MP;Dikeman DA;Mazhar M;Miller RJ;Anderson LS;Francis KP;Simon SI;Miller LS
金黄色葡萄球菌感染的体内全动物光学（生物发光和荧光）成像提供了无创和纵向监测活麻醉动物中细菌负荷动态和随后宿主免疫反应的机会。在此，我们描述了几种不同的小鼠模型的S。金黄色葡萄球菌皮肤感染、皮肤炎症、切口/切除伤口感染以及矫形植入物感染的小鼠和兔模型，其利用了该成像技术。这些动物模型和成像方法提供了对这些感染和先天性和适应性免疫应答的发病机制的见解，以及诊断和治疗方式的临床前评价。非侵入性的方法来研究宿主-病原体相互作用是非常重要的，因为有毒的社区获得性耐甲氧西林沙门氏菌。金黄色葡萄球菌菌株（CA-MRSA）正在正常人群中传播，变得越来越耐抗生素，并对公共卫生造成严重威胁。
In vivo whole-animal optical (bioluminescence and fluorescence) imaging of Staphylococcus aureus infections has provided the opportunity to noninvasively and longitudinally monitor the dynamics of the bacterial burden and ensuing host immune responses in live anesthetized animals. Herein, we describe several different mouse models of S. aureus skin infection, skin inflammation, incisional/excisional wound infections, as well as mouse and rabbit models of orthopedic implant infection, which utilized this imaging technology. These animal models and imaging methodologies provide insights into the pathogenesis of these infections and innate and adaptive immune responses, as well as the preclinical evaluation of diagnostic and treatment modalities. Noninvasive approaches to investigate host-pathogen interactions are extremely important as virulent community-acquired methicillin-resistant S. aureus strains (CA-MRSA) are spreading through the normal human population, becoming more antibiotic resistant and creating a serious threat to public health.