PRESENCE AND PERSISTENCE OF FOOT-AND-MOUTH DISEASE VIRUS IN BOVINE SKIN
PRESENCE AND PERSISTENCE OF FOOT-AND-MOUTH DISEASE VIRUS IN BOVINE SKIN
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DOI：
10.1128/jb.91.6.2333-2338.1966
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发表时间：
1966-01-01
影响因子：
3.2
通讯作者：
COTTRAL, GE
中科院分区：
文献类型：
--
作者：
GAILIUNAS, P;COTTRAL, GE
这项研究确定了口蹄疫病毒（FMDV）的七种已知抗原类型对牛皮肤的所有区域都具有一致的亲和力，尽管肉眼皮肤病变通常仅在足部发现。大量的口蹄疫病毒存在于13个不同身体部位的皮肤中，无论是否存在毛发。从50头实验感染的阉牛的躯干上采集的所有皮肤标本，在接种后12小时至7天（DPI）进行尸检，在真皮和表皮组织中含有FMDV。在一些阉牛的皮肤中，FMDV在病毒血症停止后持续长达5天。最高的平均病毒滴度，103.6空斑形成单位（PFU）每克皮肤，发现在2 DPL的躯干和四肢的一些地区有滴度约105.0 PFU每克皮肤。在取样区未观察到特征性肉眼病变。目前的观察结果对国际贸易中提供的生皮具有流行病学重要性，因为口蹄疫病毒局限于皮内比病毒粘附于生皮表面更难清除。
This study established that the seven known antigenic types of foot-and-mouth disease virus (FMDV) have consistent affinity to all areas of bovine skin, even though gross cutaneous lesions usually are found only in the pedal area. Considerable amounts of FMDV were present in skin of 13 different body areas, irrespective of the presence of hair. All skin specimens from the trunk of 50 ex- perimentally infected steers, necropsied from 12 hr to 7 days post-inoculation (DPI), contained FMDV in the dermal and epidermal tissues. In skins of some steers, FMDV persisted for as long as 5 days after cessation of viremia. The highest average virus titer, 103.6 plaque-forming units (PFU) per g of skin, was found at 2 DPL Some areas of the trunk and extremities had titers of approximately 105.0 PFU per g of skin. Characteristic gross lesions were not observed in sampling areas. The present observations have epizootiological importance for hides offered in international trade, because FMDV localized intracutaneously is more difficult to inactivate than virus adhering to hide surfaces.