Multilocus genotyping of potentially zoonotic Giardia duodenalis in pet chinchillas (Chinchilla lanigera) in China
Multilocus genotyping of potentially zoonotic Giardia duodenalis in pet chinchillas (Chinchilla lanigera) in China
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DOI：
10.1016/j.vetpar.2015.02.001
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发表时间：
2015-03-15
影响因子：
2.6
通讯作者：
Zhang, Longxian
中科院分区：
文献类型：
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作者：
Qi, Meng;Yu, Fuchang;Zhang, Longxian
贾第鞭毛虫是一种常见的原生动物，在小肠中定居和繁殖，引起贾第鞭毛虫病。这种寄生虫感染范围广泛的脊椎动物宿主，包括人类，家畜和野生动物。有研究表明，家养的毛丝鼠是人畜共患G.尿道炎本研究对中国四个城市的140份毛丝鼠样本进行了G.尿道炎38只（27.1%）毛丝鼠G.尿道炎分析了感染率与采集地点、年龄和性别的关系。对38个龙猫样品进行了分子鉴定，以确定共同的基因型。G.通过小亚基核糖体RNA（ssur RNA）基因的分析，在毛丝鼠样品中鉴定出A和B两个亚群。使用多个基因（谷氨酸脱氢酶（gdh），磷酸丙糖异构酶（tpi）和β-贾第丁（bg）基因）在亚型水平上进行基因分型，确定了大多数组合A分离物序列与亚型AI相同。组装B分离株属于亚型BIV的核苷酸序列之间的变异性。这是G.中国龙猫中的黄毛菊。由于AT和BIV亚型与人类感染有关，G.毛丝鼠的寄生虫应视为人畜共患。(C)2015 Elsevier B. V.版权所有。
Giardia duodenalis is a common protozoan that colonizes and reproduces in the small intestine, causing giardiasis. This parasite infects a wide range of vertebrate hosts, including humans, domestic animals and wildlife. It has been suggested that chinchillas (Chinchilla lanigera) kept as domestic pets are potential reservoirs for the zoonotic transmission of G. duodenalis. In this study, 140 chinchilla samples from four cities in China were examined to determine the prevalence of G. duodenalis. Thirty-eight (27.1%) chinchillas were found to be positive for G. duodenalis. The prevalence of infection was analyzed in relation to collection site, age and sex. Molecular characterization was also carried out on the 38 chinchilla samples to determine common genotypes. G. duodenalis assemblages A and B were identified in the chinchilla samples by analysis of the small subunit ribosomal RNA (ssur RNA) gene. Genotyping at the subtype level using multiple genes (glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi) and beta-giardin (bg) genes) determined that the majority of assemblage A isolate sequences were identical to subtype AI. Assemblage B isolates showed variability among the nucleotide sequences belonging to subtype BIV. This is the first report of G. duodenalis in chinchillas from China. As subtype AT and BIV are associated with human infection, G. duodenalis in chinchillas should be regarded as zoonotic. (C) 2015 Elsevier B.V. All rights reserved.