A YEAST PROTEIN THAT INFLUENCES THE CHROMATIN STRUCTURE OF UASG AND FUNCTIONS AS A POWERFUL AUXILIARY GENE ACTIVATOR
A YEAST PROTEIN THAT INFLUENCES THE CHROMATIN STRUCTURE OF UASG AND FUNCTIONS AS A POWERFUL AUXILIARY GENE ACTIVATOR
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DOI：
10.1101/gad.4.4.503
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发表时间：
1990-04-01
影响因子：
10.5
通讯作者：
KORNBERG, RD
中科院分区：
文献类型：
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作者：
CHASMAN, DI;LUE, NF;KORNBERG, RD
GRF2，Mr.apprx 丰富的酵母蛋白。 127,000，与 GAL 上游激活序列 (UASG) 结合并创建 .apprx 的无核小体区域。 230bp。纯化的 GRF2 与许多其他 UAS、35S rRNA 增强子、着丝粒和端粒中发现的序列结合。尽管 GRF2 本身仅轻微刺激转录，但它与邻近的弱激活剂结合可提供高达 170 倍的增强。 GRF2 的这种效应强烈依赖于距离，当 GRF2 和邻近激活位点之间插入 22 bp 时，该效应下降 85%。
GRF2, an abundant yeast protein of Mr .apprx. 127,000, binds to the GAL upstream activating sequence (UASG) and creates a nucleosome-free region of .apprx. 230 bp. Purified GRF2 binds to sequences found in many other UASs, in the 35S rRNA enhancer, at centromeres, and at telomeres. Although GRF2 stimulates transcription only slightly on its own, it combines with a neighboring weak activator to give as much as a 170-fold enhancement. This effect of GRF2 is strongly distance-dependent, declining by 85% when 22 bp is interposed between the GRF2 and neighboring activator sites.