Boundary cells between longitudinal and circular layers: essential for electrical slow waves in cat intestine.
Boundary cells between longitudinal and circular layers: essential for electrical slow waves in cat intestine.
复制标题
DOI：
10.1152/ajpgi.1986.250.3.g287
复制
发表时间：
1986-03
期刊：
影响因子：
--
通讯作者：
N. Suzuki;C. Prosser;V. Dahms
中科院分区：
文献类型：
--
作者：
N. Suzuki;C. Prosser;V. Dahms
用细胞内电极、准细胞内压力电极和不同水平的肌肉纤维吸电极记录了猫空肠边缘制剂中的慢波电。纵向肌层和圆形肌层的同时记录显示，边界区附近的任一肌肉层的静息电位相似，而粘膜下层附近的圆形肌细胞的静息电位较低。慢波在两层的边界处振幅最大，并在两层的边界处以电张力向外扩散。双极记录在边界两侧具有相反的极性。内圆纤维慢波振幅明显低于外圆纤维慢波振幅。用亚甲基蓝染色法鉴定有或没有Cajal丛间质细胞附着的肌层小条。记录后观察到Cajal间质细胞（ICC）的区域出现慢波。在未观察到ICC的区域，两层均未记录到慢波。含有ICC但不含ICC的条带可以电驱动。由于神经元的阻断并不能消除慢波，并且由于缺乏ICC的肌肉区域没有慢波，因此可以得出结论，间质细胞（ICC- i）最有可能是肠肌两层慢波所必需的边界元素。
Electrical slow waves were recorded by intracellular electrodes and by quasi-intracellular pressure and suction electrodes from muscle fibers at different levels in edgewise preparations of cat jejunum. Simultaneous recordings from longitudinal and circular muscle layers showed similar resting potentials from either muscle layer near the boundary zone, and lower resting potentials in cells of circular muscle near the submucosa. Slow waves were maximal in amplitude at the boundary between the two layers and spread electrotonically away from the boundary in both layers. Bipolar recordings were of opposite polarity on the two sides of the boundary. Amplitudes of slow waves from inner circular fibers were significantly lower than from outer circular fibers. Small strips of each muscle layer were prepared with or without the attached interstitial cells of Cajal plexus as identified by methylene blue staining. Either muscle layer showed slow waves from regions where interstitial cells of Cajal (ICC) were observed after the recording. No slow waves were recorded from either layer from regions where ICC were not observed. Strips containing ICC but not strips lacking ICC could be driven electrically. Since blocking of neurons does not abolish slow waves and since regions of muscle lacking ICC do not have slow waves, it is concluded that the interstitial cells (ICC-I) are most likely the boundary elements essential for slow waves in either layer of intestinal muscle.