Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture
Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture
复制标题
克隆衍生的人类胚胎干细胞系在长期培养过程中保持多能性和增殖潜力
DOI：
10.1006/dbio.2000.9912
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发表时间：
2000-11-15
影响因子：
2.7
通讯作者：
Thomson, JA
中科院分区：
文献类型：
--
作者：
Amit, M;Carpenter, MK;Thomson, JA
源自人类囊胚的胚胎干（ES）细胞系即使经过长时间培养，仍具有发育成所有三个胚胎胚层衍生物的潜能。在此我们描述了两个人类ES细胞系H9.1和H9.2的克隆衍生过程。在H9.1和H9.2 ES细胞系克隆衍生时，其亲本ES细胞系H1已经连续培养了6个月。经过另外8个月的培养，H9.1和H9.2 ES细胞系继续：（1）活跃增殖，（2）高水平表达端粒酶，以及（3）保持正常核型。端粒长度虽然有些变化，但在高代次的H9.1和H9.2细胞中保持在8到12kb之间。高代次的H9.1和H9.2细胞在SCID - beige小鼠中均形成了畸胎瘤，其中包含所有三个胚胎胚层的分化衍生物。这些结果证明了单个人类ES细胞的多能性、在长时间培养过程中多能性的维持以及培养的人类ES细胞的长期自我更新特性。人类ES细胞显著的发育潜能、增殖能力和核型稳定性使其有别于成年细胞。（C）2000学术出版社
需注意原文中“the parental ES cell line, Ho”疑似应为“H1”，翻译时按正确逻辑进行了处理，如果原文无误请根据实际情况调整。
Embryonic stem (ES) cell lines derived from human blastocysts have the developmental potential to form derivatives of all three embryonic germ layers even after prolonged culture. Here we describe the clonal derivation of two human ES cell lines, H9.1 and H9.2. At the time of the clonal derivation of the H9.1 and H9.2 ES cell lines, the parental ES cell line, Ho, had already been continuously cultured for 6 months. After an additional 8 months of culture, H9.1 and H9.2 ES cell lines continued to: (1) actively proliferate, (2) express high levels of telomerase, and (3) retain normal karyotypes. Telomere lengths, while somewhat variable, were maintained between 8 and 12 kb in high-passage H9.1 and H9.2 cells. High-passage H9.1 and H9.2 cells both formed teratomas in SCUD-beige mice that included differentiated derivatives of all three embryonic germ layers. These results demonstrate the pluripotency of single human ES cells, the maintenance of pluripotency during an extended period of culture, and the long-term self-renewing properties of cultured human ES cells. The remarkable developmental potential, proliferative capacity, and karyotypic stability of human ES cells distinguish them from adult cells. (C) 2000 Academic Press.