Tubular changes in obstructed kidney of adult mice evaluated using immunohistochemistry for segment-specific marker
Tubular changes in obstructed kidney of adult mice evaluated using immunohistochemistry for segment-specific marker
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DOI：
10.14670/hh-22.291
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发表时间：
2007-03-01
影响因子：
2
通讯作者：
Sato, T.
中科院分区：
文献类型：
--
作者：
Kida, Y.;Sato, T.
本研究的主要目的是应用节段性肾小管标志物检测成年小鼠单侧输尿管梗阻(UUO)模型的梗阻肾脏，并对慢性肾脏疾病的终末期结局--肾小管间质纤维化的典型模型--UUO进行详细的形态学评价。成年小鼠行UUO，分别于术后1、3、7d取肾脏。HE和AZN染色证实UUO后3d皮质和髓质均有间质间隙扩张。间质纤维化主要发生在扩张的小管周围。节段特异性抗体免疫组织化学检测显示，近端小管和Henle‘s环降支在UUO后7天才开始扩张，而Henle’s环上升肢在术后1天才开始扩张。从远端小管至集合管的节段在术后1天开始扩张，之后明显扩张。本组梗阻肾中，肾单位下游参与扩张早于肾单位上游。此外，伴有肾小管上皮细胞凋亡的肾小管较未见凋亡的肾小管明显扩张。上述结果提示，肾单位远端(从Henle‘s环升支至集合管)的肾小管扩张可导致尿蓄积所致的肾小管上皮细胞凋亡，这可能与成年小鼠UUO肾小管消失并被纤维组织替代有关。
The main focus of the present investigation is to examine obstructed kidneys due to unilateral ureteral obstruction ( UUO) model in adult mice using segment-specific tubular marker and to confirm the detailed morphological evaluation of UUO that is a typical model for the tubulointerstitial fibrosis which is an endpoint outcome of chronic renal diseases. Adult mice were subjected to UUO, and kidneys were harvested 1, 3, 7 days after surgical operation. Expansion of interstitial space both in the cortex and the medulla was confirmed 3 days after UUO by HE- and azan- staining. Interstitial fibrosis developed especially around dilated tubules. Immunohistochemistry for segment- specific antibodies revealed that the proximal tubules and the descending limb of Henle's loop did not dilate until 7 days after UUO, whereas initial dilation of the ascending limb of Henle's loop appeared to occur one day after surgery. The segment from the distal tubules to the collecting ducts began dilating one day after surgery and afterward significantly dilated. The downstream segment of nephron was involved in dilating earlier than the upstream of nephron in obstructed kidney examined in the present study. Moreover, the tubules accompanying apoptosis of tubular epithelia significantly dilated compared with those without apoptotic tubular epithelia. From the above- mentioned findings, we conclude that tubular dilatation of distal segment ( from the ascending limb of Henle's loop to the collecting ducts) of nephron develops tubular epithelial apoptosis caused by accumulated urine, which would link to tubular disappearance and its replacement with fibrous tissue in UUO kidney of adult mice.