Potential utilization of agro-industrial wastewaters for lipid production by the oleaginous yeast Debaryomyces etchellsii
Potential utilization of agro-industrial wastewaters for lipid production by the oleaginous yeast Debaryomyces etchellsii
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DOI：
10.1016/j.jclepro.2016.06.040
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发表时间：
2016-10-01
影响因子：
11.1
通讯作者：
Mechichi, Tahar
中科院分区：
文献类型：
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作者：
Arous, Fatma;Frikha, Fakher;Mechichi, Tahar
研究了产油酵母Debarymyces etchellsii菌株BM1在去除污染负荷的同时，将脱蛋白乳清、橄榄磨废水、糖果工业废水和过期软饮料等多种低成本碳源转化为单细胞油脂的能力。有趣的是，该菌株能够生长并积累大量的储备脂肪(14.7-25.9wt/wt干生物量)，其脂肪酸组成与目前用于生物柴油制造的植物油相似，如棕榈油和高油酸葵花籽油。从不同底物生产的生物柴油符合欧洲标准EN 14214对十六烷值、碘值和冷滤器堵点等关键参数的限制。以过期软饮料、橄榄磨废水(水中体积比为25%)和脱蛋白奶酪乳清为主要原料，采用单纯形-质心混合设计法对发酵条件进行了优化。模型估计，当过期软饮料和橄榄磨废水(水中含量为25%，v/v)按62.4%和37.6%的比例混合作为底物时，干生物量中脂肪含量最高可达28.1%wt/wt，脂肪得率为1.2g/L，细胞质量得率为4g/L。以50：50(v/v)的干酪乳清和过期软饮料组成的培养基产7.9g/L干生物量，含脂量15.1%，产脂率相同(1.2g/L)。在该介质中，观察到化学需氧量显著降低(约58%)。本研究为生物柴油的同时回收和废水处理提供了一条有效的途径。(C)2016爱思唯尔有限公司。保留所有权利。
The present study was carried out in order to investigate the ability of the oleaginous yeast Debaryomyces etchellsii strain BM1 to convert a variety of low-cost carbon sources, i.e. deproteinized cheese whey, olive mill wastewater, wastewaters from confectionary industries and expired soft drinks, into single cell oil with simultaneous pollution load removal. Interestingly, this strain was able to grow and to accumulate significant quantities of reserve lipids (14.7-25.9% wt/wt in dry biomass) of similar fatty acid composition to that of vegetable oils currently used in the biodiesel manufacture such as palm and high oleic sunflower oils. Biodiesel produced from different substrates satisfied the limits imposed by the European Standard EN 14214 for critical parameters like cetane number, iodine value and cold filter plugging point. Medium optimization was performed using simplex-centroid mixture design method based on three media components, i.e. expired soft drinks, olive mill wastewaters (25%, v/v in water) and deproteinized cheese whey. The model estimated that a maximum lipid content of 28.1% wt/wt in dry biomass, a lipid yield of 1.2 g/L and a cell mass yield of 4 g/L could be obtained when a mixture of expired soft drinks and olive mill wastewater (25% in water, v/v) is used as substrate at percentages 62.4% and 37.6%, respectively. On the other hand, a medium containing cheese whey and expired soft drinks at 50:50 (v/v) yielded 7.9 g/L dry biomass containing 15.1% lipids, leading to the same lipid yield (1.2 g/L). In this medium, a significant reduction (around 58%) of the chemical oxygen demand was observed. This research provides an efficient approach for simultaneous biodiesel recovery and wastewater treatment. (C) 2016 Elsevier Ltd. All rights reserved.