A CRITICAL ROLE OF LAMBDA-5 PROTEIN IN B-CELL DEVELOPMENT
A CRITICAL ROLE OF LAMBDA-5 PROTEIN IN B-CELL DEVELOPMENT
复制标题
DOI：
10.1016/0092-8674(92)90293-l
复制
发表时间：
1992-05-29
期刊：
影响因子：
64.5
通讯作者：
RAJEWSKY, K
中科院分区：
文献类型：
--
作者：
KITAMURA, D;KUDO, A;RAJEWSKY, K
Lambda-5基因是免疫球蛋白J(Lambda)-C(Lambda)基因的同源物，在未成熟的B系细胞中特异表达。Lambda-5编码的分子与Mu或D(Mu)蛋白形成膜复合体，与V(Pre-B)基因编码的另一种在未成熟B细胞中特异表达的蛋白质结合在一起。我们已经产生了通过胚胎干细胞中的靶向基因破坏而使lambda-5基因失活的小鼠。在这些小鼠中，骨髓中的B细胞发育在前B细胞阶段受阻。然而，这种封锁是有漏洞的，允许B细胞以较低的速度进入外周免疫系统。这些细胞被等位基因排除，并能够对抗原做出反应。
The lambda-5 gene is a homolog of immunoglobulin J(lambda)-C(lambda) genes, expressed specifically in immature B-lineage cells. Lambda-5-encoded molecules form membrane complexes with mu or D(mu) proteins in association with an additional protein specifically expressed in immature B cells that is encoded by the V(pre-B) gene. We have generated mice in which the lambda-5 gene is inactivated by targeted gene disruption in embryonic stem cells. In these mice, B cell development in the bone marrow is blocked at the pre-B cell stage. However, the blockade is leaky, allowing B cells to populate the peripheral immune system at a low rate. These cells are allelically excluded and able to respond to antigen.