AP-2.2, a novel gene related to AP-2, is expressed in the forebrain, limbs and face during mouse embryogenesis
AP-2.2, a novel gene related to AP-2, is expressed in the forebrain, limbs and face during mouse embryogenesis
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DOI：
10.1016/0925-4773(95)00463-7
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发表时间：
1996-01-01
影响因子：
2.6
通讯作者：
Dolle, P
中科院分区：
文献类型：
--
作者：
Chazaud, C;OuladAbdelghani, M;Dolle, P
使用差减法杂交克隆程序，我们最近发现AP-2.2基因是一个新的早期维甲酸诱导基因在小鼠P19胚胎癌细胞。我们还发现与AP-2转录因子高度相关的AP-2.2蛋白在与AP-2一致结合位点结合时可以激活转录[Oulad-Abdelghani et al. (1995) Mel。细胞。医学杂志。,提交]。我们在此报告了AP-2.2转录物在小鼠胚胎发生过程中的原位杂交表达模式。在交配后7.5 d，在神经板和表面外胚层之间的边界区域以及胚胎外组织中检测到AP-2.2转录本。在妊娠8.0-8.5天，AP-2.2转录本在预迁移和迁移的神经嵴细胞中表达。在接下来的几天里，AP-2.2基因在面部间质中表现出区域限制性表达，特别是在胚胎口腔和鼻腔周围，以及表面外胚层、鼻和口腔上皮。AP-2.2 RNA也在假定的前脑囊泡皮质区特异性表达。AP-2.2转录本局限于肢芽和生殖芽的远端有丝分裂区（“进展区”）。AP-2.2的表达似乎也特异于生殖嵴的原始生殖细胞。因此，AP-2.2基因在几个胚胎区域表达，这些区域的发育可能受到类维生素a的影响，如前脑、面部和肢体芽。
Using a differential subtractive hybridization cloning procedure we have recently identified the AP-2.2 gene as a novel early retinoic acid-induced gene in murine P19 embryonal carcinoma cells. We have also shown that the AP-2.2 protein, which is highly related to the AP-2 transcription factor, can activate transcription when bound to an AP-2 consensus binding site [Oulad-Abdelghani et al. (1995) Mel. Cell. Biol., submitted]. We report here the in situ hybridization pattern of expression of AP-2.2 transcripts during mouse embryogenesis. At 7.5 days post-coitum, AP-2.2 transcripts were detected in the boundary region between neural plate and surface ectoderm, as well as in extra-embryonic tissues. By 8.0-8.5 gestational days, AP-2.2 transcripts appeared to be expressed in premigratory and migrating neural crest cells. Over the following days, the AP-2.2 gene displayed region-restricted expression in the facial mesenchyme, especially around the embryonic mouth cavity and the nasal cavities, as well as in the surface ectoderm, nasal and oral epithelia. AP-2.2 RNA was also specifically expressed in the presumptive cortical region of the forebrain vesicles. AP-2.2 transcripts were restricted to the distal mitotic area (the 'progress zone') of the limb buds and of the genital bud. AP-2.2 expression also appeared to be specific for primordial germ cells in the genital ridges. Thus, the AP-2.2 gene is expressed in several embryonic areas whose development can be affected by retinoids, such as the forebrain, face and limb buds.