Morphine upregulates mu opioid receptors of human and monkey lymphocytes.
Morphine upregulates mu opioid receptors of human and monkey lymphocytes.
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吗啡上调人和猴淋巴细胞的μ阿片受体。
DOI：
10.1006/bbrc.2000.4006
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发表时间：
2000
影响因子：
3.1
通讯作者：
Chuang,RY
中科院分区：
文献类型：
--
作者：
Suzuki,S;Miyagi,T;Chuang,TK;Chuang,LF;Doi,RH;Chuang,RY
免疫细胞中也发现了类似于脑细胞中发现的阿片受体 delta、kappa 和 mu 亚型。目前的研究表明，通过竞争性聚合酶链反应，用吗啡处理人淋巴细胞会导致μ阿片受体基因表达量增加。使用针对 MOR-1（神经元 mu 阿片受体）的抗体对 mu 蛋白进行蛋白质印迹分析，结果显示一条约 50 kDa 的单条带，吗啡处理后其强度增加。当用吗啡处理猴子淋巴细胞时，观察到μ阿片受体激活的类似结果。这些研究表明，除了通过与神经内分泌系统通讯产生免疫效应外，精神活性药物吗啡还可以通过直接作用于淋巴细胞上表达的μ阿片受体来调节免疫功能。
Opioid receptors of subtypes delta, kappa, and mu similar to those found in brain cells have been identified in immune cells. The current study demonstrates by competitive polymerase chain reaction the treatment of human lymphocytic cells with morphine resulting in an increased amount of gene expression of mu opioid receptors. Antibodies against the MOR-1, the neuronal mu opioid receptor, were used in Western blot analysis of mu proteins and the results revealed a single band of ∼50 kDa, the intensity of which was increased by morphine treatment. Similar results of mu opioid receptor activation were observed when monkey lymphocytes were treated with morphine. These studies suggest that in addition to causing an immune effect through communication with the neuroendocrine system, the psychoactive drug morphine may modulate immune functions by acting directly on the mu opioid receptors expressed on lymphocytes.