RELEASE OF NEUROPEPTIDES DURING INTRACELLULAR STIMULATION OF SINGLE IDENTIFIED APLYSIA NEURONS IN CULTURE
RELEASE OF NEUROPEPTIDES DURING INTRACELLULAR STIMULATION OF SINGLE IDENTIFIED APLYSIA NEURONS IN CULTURE
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DOI：
10.1073/pnas.83.24.9794
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发表时间：
1986-12-01
影响因子：
11.1
通讯作者：
WEISS, KR
中科院分区：
文献类型：
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作者：
LLOYD, PE;SCHACHER, S;WEISS, KR
将一种物质分类为神经递质的一个重要标准是它以活动依赖的方式释放。我们利用小心脏活性肽A和小心脏活性肽B对小心脏活性肽SCPA和SCPB的释放进行了细胞培养。从海兔颊神经节中分离出神经元B1和B2，进行细胞培养。这些细胞生长出新的突起，对神经肽SCPB抗体有免疫反应。这些过程中含有SCPA和SCPB，可通过蜗牛心脏生物测定法检测到。细胞从放射性标记的前体合成scp，并将肽运送到它们的神经突。单细胞在细胞内电刺激下以钙依赖的方式释放scp。综上所述，这些结果提供了关键证据，证明scp是神经递质。结果还表明，单个鉴定神经元的细胞培养可用于研究肽的释放。
An important criterion for classifying a substance as a neurotransmitter is that it is released in an activity-dependent fashion. We have utilized cell culture of individual neurons of Aplysia to demonstrate the release of the neuropeptides SCPA and SCPB (small cardioactive peptides A and B). Neurons B1 and B2 were isolated from the buccal ganglion of Aplysia and maintained in cell culture. The cells grew new processes, which were immunoreactive to antibodies for the neuropeptide SCPB. These processes contained SCPA and SCPB that were detectable by bioassay on snail heart. The cells synthesized the SCPs from radiolabeled precursors and transported the peptides to their neurites. Single cells released SCPs in a calcium-dependent fashion upon intracellular electrical stimulation. Taken together, these results provide critical evidence that SCPs are neurotransmitters. The results also indicate that the cell culture of individual identified neurons can be used to investigate the release of peptides.