MONOFUNCTIONAL ADDUCTS OF PLATINUM(II) PRODUCE IN DNA A SEQUENCE-DEPENDENT LOCAL DENATURATION
MONOFUNCTIONAL ADDUCTS OF PLATINUM(II) PRODUCE IN DNA A SEQUENCE-DEPENDENT LOCAL DENATURATION
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DOI：
10.1021/bi00172a005
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发表时间：
1994-02-15
期刊：
影响因子：
2.9
通讯作者：
BALCAROVA, Z
中科院分区：
文献类型：
--
作者：
BRABEC, V;BOUDNY, V;BALCAROVA, Z
用单链特异性探针氯乙醛（CAA）研究了氯代（二乙烯三胺）氯化铂（II）或顺式二胺水合氯代铂（II）的单官能加合物对DNA构象的影响。CAA结合的变性位点，并诱导在DNA中的铂络合物的单功能加合物，其特征在于通过三种实验方法。这些包括测量用CAA处理的质粒片段的荧光，分析用CAA处理并被哌啶切割的寡核苷酸，以及终止用CAA处理的质粒DNA片段上的双链体转录。结果表明，在三核苷酸序列Py-脱氧鸟苷-Py（Py是嘧啶脱氧核苷）中，变性优先发生在含有单加合脱氧鸟苷的碱基对上。有人建议，这种构象的改变有利于在DNA中形成轻微的双功能加合物的顺二氨二氯铂（II）。
The effects on the conformation of DNA produced by the monofunctional adducts of chloro(diethylenetriamine)platinum(II) chloride or cis-diamminemonoaquamonochloroplatinum(II) have been investigated by means of the single-strand-specific probe chloroacetaldehyde (CAA). The denatured sites to which CAA was bound and that were induced in DNA by the monofunctional adducts of the platinum complexes were characterized by means of three experimental approaches. These include measurement of the fluorescence of a plasmid fragment treated with CAA, analysis of oligonucleotides treated with CAA and cleaved by piperidine, and termination of duplex transcription on a fragment of plasmid DNA treated with CAA. The results indicate that the denaturational change preferentially occurs in the base pair containing the monoadducted deoxyriboguanosine in the trinucleotide sequence Py-deoxyriboguanosine-Py (Py is a pyrimidine deoxyribonucleoside). It was suggested that this conformational alteration facilitates in DNA the formation of minor bifunctional adducts of cis-diamminedichloroplatinum(II).