Serologic responses of cattle and other animals infected with Neospora caninum.
Serologic responses of cattle and other animals infected with Neospora caninum.
复制标题
感染犬新孢子虫的牛和其他动物的血清学反应。
DOI：
10.2460/ajvr.1996.57.03.329
复制
发表时间：
1996
影响因子：
1
通讯作者：
P. Thulliez
中科院分区：
文献类型：
--
作者：
J. Dubey;D. Lindsay;D. S. Adams;J. Gay;T. Baszler;B. Blagburn;P. Thulliez
客观化
研究犬新孢子虫和与之密切相关的顶端复合体之间的交叉反应。
设计
检测动物血清对N犬抗体的产生和与弓形虫的交叉反应。
动物
试验用犬、弓形虫和肉孢子虫3种组织囊化原虫感染牛，用牛肠球虫、牛艾美耳球虫和微小隐孢子虫单种接种犊牛。在实验室兔身上也进行了类似的研究，接种了N犬、弓形虫、哈蒙德哈蒙迪和肉孢子虫。此外，从接种N犬速殖子的母羊、绵羊、山羊、母猪、猫、大鼠和小鼠身上获得血清。
程序
用间接荧光抗体(IFA)和酶联免疫吸附试验(仅限牛)检测对N犬的反应性，用改良的直接凝集法、Sabin-Feldman染料和IFA试验评价对弓形虫的反应性。
结果
通过IFA试验，犬N、弓形虫和肉孢子虫之间的血清学交叉反应为零或最小。用EL ISA检测接种肉孢子虫的牛体内对N犬的反应性。
结论
犬新孢子病的IFA检测对动物新孢子病的诊断具有特异性。
OBJECTIVE
To examine cross-reactivity among Neospora caninum and closely-related apicomplexans.
DESIGN
Sera from animals were examined for antibody production to N caninum and cross-reactivity to Toxoplasma gondii.
ANIMALS
Cattle were experimentally infected with 3 tissue cyst-forming protozoan parasites N caninum, T gondii, and Sarcocystis sp, and calves were monospecifically inoculated with the intestinal coccidia, Eimeria bovis and Cryptosporidium parvum. Similar studies were done in laboratory rabbits inoculated with N caninum, T gondii, Hammondia hammondi, and Sarcocystis sp. Additionally, sera were obtained from ewes, lambs, goats, sows, cats, rats, and mice inoculated with N caninum tachyzoites.
PROCEDURE
The indirect fluorescent antibody (IFA) and ELISA antibody tests (cattle only) were used to examine reactivity to N caninum; the modified direct agglutination, Sabin-Feldman dye, and IFA tests were used to evaluate reactivity to T gondii.
RESULTS
Serologic cross-reactivity among N caninum, T gondii, and Sarcocystis sp was none or minimal by the IFA test. There was some reactivity to N caninum by the use of ELISA in cattle inoculated with Sarcocystis sp.
CONCLUSIONS
The IFA test for N caninum was specific for the diagnosis of neosporosis in animals.