The oxidative state of chylomicron remnants influences their modulation of human monocyte activation.
The oxidative state of chylomicron remnants influences their modulation of human monocyte activation.
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DOI：
10.1155/2012/942512
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发表时间：
2012
影响因子：
1.3
通讯作者：
Lawson C
中科院分区：
文献类型：
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作者：
Armengol Lopez S;Botham KM;Lawson C
乳糜微粒残余物（CMRs）通过增加活性氧（ROS）的产生和细胞迁移，直接促进体外人单核细胞活化。本研究探讨了CMR氧化状态对单核细胞活化程度的影响。cmr样颗粒（CRLPs）在三种不同的氧化状态下制备，正常（CRLPs），通过加入抗氧化剂普罗布科尔（pCRLPs）来防止氧化，或与CuSO4氧化（oxCRLPs）。在与CRLPs孵育的原代人单核细胞中，脂质积累和ROS产生显著增加，而单核细胞化学引诱蛋白-1的分泌减少，但oxCRLPs没有额外的影响。相比之下，pCRLPs被单核细胞摄取的程度较小，对ROS或MCP-1的分泌没有显著影响。这些研究表明，cmr的氧化状态调节了它们对人外周血单核细胞活化的刺激，饮食抗氧化剂可能对这些动脉粥样硬化作用提供了一定的保护。
Chylomicron remnants (CMRs) contribute directly to human monocyte activation in vitro, by increasing reactive oxygen species (ROS) production and cell migration. In this study, the effects of the oxidative state of CMR on the degree of monocyte activation was investigated. CMR-like particles (CRLPs) were prepared in three different oxidative states, normal (CRLPs), protected from oxidation by incorporation of the antioxidant, probucol (pCRLPs), or oxidised with CuSO4 (oxCRLPs). Lipid accumulation and ROS production were significantly increased in primary human monocytes incubated with CRLPs, whilst secretion on monocyte chemoattractant protein-1 was reduced, but oxCRLPs had no additional effect. In contrast, pCRLPs were taken up by monocytes to a lesser extent and had no significant effect on ROS or MCP-1 secretion. These studies suggest that the oxidative state of CMRs modulates their stimulation of the activation of peripheral blood human monocytes and that dietary antioxidants may provide some protection against these atherogenic effects.