Effect of cholinergic agonists on muscle from rodent proximal and distal small intestine.
Effect of cholinergic agonists on muscle from rodent proximal and distal small intestine.
复制标题
胆碱能激动剂对啮齿动物近端和远端小肠肌肉的影响。
DOI：
10.1016/s0016-5085(85)80069-x
复制
发表时间：
1985
期刊：
影响因子：
29.4
通讯作者：
Harrington,B
中科院分区：
文献类型：
--
作者：
Nowak,TV;Harrington,B
将近端和远端大鼠小肠切成6.0 × 10.0 mm的条带。沿口尾轴沿着切割的条带称为纵向条带，而与该轴成90°切割的条带称为环形条带。连续测量环形和纵向肌条的应力，因为它们与乙酰胆碱，氨甲酰胆碱，乙酰甲胆碱，氨甲酰胆碱，或毒扁豆碱superf使用。在拉伸，乙酰胆碱刺激的主动应力，和总应力期间的休息应力进行了测定。近端环行肌对乙酰胆碱的敏感性是远端环行肌的5倍（p < 0.05），近端纵行肌对氨甲酰胆碱的敏感性是远端纵行肌的2.8倍（p < 0.05）。近端和远端肌肉的静息应力、活动应力和总应力相似，但环形肌的近端或远端部位的静息应力几乎是纵向肌的两倍（p < 0.05）。毒扁豆碱（10- 6 M）使近端和远端环行肌的乙酰胆碱刺激的主动应激分别增加29%和70%（p < 0.05），但对纵行肌无明显影响（p > 0.05）。环肌近端和远端的这一差异（41%）也是显著的（p < 0.05）。因此，近端和远端肌肉的大鼠小肠不同的敏感性，各种胆碱能激动剂，但不是在其长度应力特性。
Proximal and distal rat small intestine was cut into strips measuring 6.0 × 10.0 mm. Strips cut along the oral-caudal axis were called longitudinal strips, whereas those cut 90° to that axis were called circular strips. Stress in circular and longitudinal muscle strips was measured continuously as they were superf used with acetylcholine, carbamylcholine, methacholine, bethanechol, or physostigmine. Resting stress during stretch, acetylcholine-stimulated active stress, and total stress were determined. Proximal circular muscle was five times as sensitive to acetylcholine as distal circular muscle (p < 0.05); proximal longitudinal muscle was 2.8 times as sensitive to bethanechol as distal muscle (p < 0.05). Resting, active, and total stress were similar in proximal and distal muscle, but circular muscle showed nearly twice the resting stress of longitudinal muscle at either proximal or distal sites (p < 0.05). Physostigmine (10-6M) increased acetylcholine-stimulated active stress in proximal and distal circular muscle by 29% and 70%, respectively (p < 0.05), but not in longitudinal muscle (p > 0.05). This difference between proximal and distal circular muscle (41%) was also significant (p < 0.05). Thus, the proximal and distal muscle of the rat small intestine differs in its sensitivity to various cholinergic agonists, but not in its length-stress properties.