Cloning and subcellular localization of the phosphoprotein and nucleocapsid proteins of Potato yellow dwarf virus, type species of the genus Nucleorhabdovirus
Cloning and subcellular localization of the phosphoprotein and nucleocapsid proteins of Potato yellow dwarf virus, type species of the genus Nucleorhabdovirus
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DOI：
10.1016/j.virusres.2008.02.003
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发表时间：
2008-07-01
期刊：
影响因子：
5
通讯作者：
Goodin, Michael M.
中科院分区：
文献类型：
--
作者：
Ghosh, Debasish;Brooks, Robert E.;Goodin, Michael M.
我们克隆了马铃薯黄矮病毒（PYDV）sanguinolenta株系磷蛋白（P）和核衣壳（N）基因的mRNA，并对其进行了表征。P和N信使RNA都开始于一个共同的AAACA五核苷酸，长度分别为1546 nt和962 nt，能够分别编码52 kDa和31 kDa的蛋白质。NmRNA含有一个12 nt的51非翻译序列（NTS）和一个83 nt的3 '-NTS。类似地，P mRNA具有19 nt的5 '-NTS和125 nt的3'-NTS。一级结构分析显示，在P蛋白和N蛋白的6个潜在的磷酸化位点。尽管这两种蛋白质都缺乏可预测的核定位信号（NLS），但P和N蛋白在N。benthamiana表明这两种蛋白质都专门靶向细胞核。系统进化分析表明，PYDV与玉米花叶病毒和芋头脉褪绿病毒的亲缘关系最近，这两种病毒的N蛋白也缺乏可预测的NI. S。目前的数据进一步区分PYDV从SYNV，并建议，一起，这些病毒提供了一个更全面的看法弹状病毒的细胞生物学，这可以在一个共同的宿主植物进行研究。(c)2008 Elsevier B. V.保留所有权利。
We have cloned and characterized mRNAs corresponding to the phosphoprotein (P) and nucleocapsid (N) genes of the sanguinolenta strain of Potato yellow dwarf virus (PYDV). The P and N messenger RNAs both begin with a common AAACA pentanucleotide and are 1546 nt and 962 nt in length, and capable of encoding 52 kDa and 31 kDa proteins, respectively. The N mRNA contains a 12 nt 51 non-translated sequence (NTS) and a 83 nt 3'-NTS. Similarly, the P mRNA has a 19 nt 5'-NTS and a 125 nt 3'-NTS. Primary structure analyses revealed three potential phosphorylation sites in the P protein and six in the N protein. Despite a lack of predictable nuclear localization signals (NLSs) in either protein, transient expression of the P and N proteins in N. benthamiana showed that both proteins are targeted exclusively to nuclei. Phylogenetic analyses showed that PYDV is most closely related to Maize mosaic virus and Taro vein chlorosis virus, which also lack predictable NI.Ss in their N proteins. The present data further distinguish PYDV from SYNV and suggest that, together, these viruses serve to provide a more comprehensive view of rhabdovirus cell biology, which can be studied in a common host plant. (c) 2008 Elsevier B.V. All rights reserved.