Comparative transcriptome analysis of tobacco (Nicotiana tabacum) leaves to identify aroma compound-related genes expressed in different cultivated regions
Comparative transcriptome analysis of tobacco (Nicotiana tabacum) leaves to identify aroma compound-related genes expressed in different cultivated regions
复制标题
烟草（Nicotiana tabacum）叶的比较转录组分析，以确定不同栽培地区表达的香气化合物相关基因
DOI：
10.1007/s11033-012-2067-0
复制
发表时间：
2013-01-01
影响因子：
2.8
通讯作者：
Lu, Kun
中科院分区：
文献类型：
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作者：
Lei, Bo;Zhao, Xue-Hua;Lu, Kun
为了鉴定烟草中响应环境变化而差异表达的基因并解释烟草中芳香族类胡萝卜素形成的机制，采用Agilent Tobacco Gene Expression Microarray对来自中国3个栽培地区的烟草叶片进行转录组比较，开阳（KY）、威宁（WN）和天祝（TZ）。在KY和TZ的叶片中，共有1，005个基因差异表达，KY和WN之间有733个差异表达，TZ和WN之间有517个差异表达。在WN和TZ叶片中上调的基因往往参与次生代谢途径，包括几个类胡萝卜素途径基因，例如，NtPYS、NtPDS和NtLCYE，而下调的则倾向于参与对温度和光的反应。通过实时定量聚合酶链反应（qRT-PCR）评估10个差异表达基因（DEG）的表达，发现与微阵列数据一致。Gene Ontology和MapMan分析表明，在三个栽培区域中差异表达的基因与光系统II的光反应、对刺激的反应和次生代谢有关。高效液相色谱（HPLC）分析表明，来自KY的叶片具有最低水平的叶黄素、β-胡萝卜素和新黄质，而来自TZ的叶片中的总类胡萝卜素含量最高，这一发现可以很好地解释为类胡萝卜素途径中DEG的表达模式。这些结果有助于阐明烟草环境适应和香气物质积累的分子机制。
To identify genes that are differentially expressed in tobacco in response to environmental changes and to decipher the mechanisms by which aromatic carotenoids are formed in tobacco, an Agilent Tobacco Gene Expression microarray was adapted for transcriptome comparison of tobacco leaves derived from three cultivated regions of China, Kaiyang (KY), Weining (WN) and Tianzhu (TZ). A total of 1,005 genes were differentially expressed between leaves derived from KY and TZ, 733 between KY and WN, and 517 between TZ and WN. Genes that were upregulated in leaves from WN and TZ tended to be involved in secondary metabolism pathways, and included several carotenoid pathway genes, e.g.,NtPYS,NtPDS, andNtLCYE, whereas those that were down-regulated tended to be involved in the response to temperature and light. The expression of 10 differentially expressed genes (DEGs) was evaluated by real-time quantitative polymerase chain reaction (qRT-PCR) and found to be consistent with the microarray data. Gene Ontology and MapMan analyses indicate that the genes that were differentially expressed among the three cultivated regions were associated with the light reaction of photosystem II, response to stimuli, and secondary metabolism. High-performance liquid chromatography (HPLC) analysis showed that leaves derived from KY had the lowest levels of lutein, β-carotene, and neoxanthin, whereas the total carotenoid content in leaves from TZ was greatest, a finding that could well be explained by the expression patterns of DEGs in the carotenoid pathway. These results may help elucidate the molecular mechanisms underlying environmental adaptation and accumulation of aroma compounds in tobacco.