Pulsed electron paramagnetic resonance studies of the interaction of Mg-ATP and D2O with the iron protein of nitrogenase.
Pulsed electron paramagnetic resonance studies of the interaction of Mg-ATP and D2O with the iron protein of nitrogenase.
复制标题
Mg-ATP 和 D2O 与固氮酶铁蛋白相互作用的脉冲电子顺磁共振研究。
DOI：
10.1021/bi00464a026
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Peisach,J
中科院分区：
文献类型：
--
作者：
Morgan,TV;McCracken,J;Orme-Johnson,WH;Mims,WB;Mortenson,LE;Peisach,J
方法C.巴氏杆菌和棕色拟青霉是从对数生长期的300-L培养物中收获的，这些培养物生长在最少的盐介质和糖上。用Mortenson(1972)的改良方法纯化了巴氏杆菌固氮酶，按Mortenson(1972)的方法纯化了兰氏弧菌固氮酶。
MethodsC. pasteurianum and A. vinelandii were harvested from log-phase 300-L cultures grown on minimal saltmedia plus sugar. C. pasteurianum nitrogenase was purified by a mod-ification of the method of Mortenson (1972), while A. vine-landii nitrogenase was purified accordingto the method of