Biochemical Characterization of Medaka (Oryzias latipes) Transglutaminases, OlTGK1 and OlTGK2, as Orthologues of Human Keratinocyte-Type Transglutaminase.
Biochemical Characterization of Medaka (Oryzias latipes) Transglutaminases, OlTGK1 and OlTGK2, as Orthologues of Human Keratinocyte-Type Transglutaminase.
复制标题
Medaka（Oryzias latipes）转谷氨酰胺酶，OLTGK1和OLTGK2的生化表征，作为人角质形成型转谷氨酰胺酶的直系同源物。
DOI：
10.1371/journal.pone.0144194
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Hitomi K
中科院分区：
文献类型：
--
作者：
Kikuta A;Furukawa E;Ogawa R;Suganuma N;Saitoh M;Nishimaki T;Katsumura T;Oota H;Kawamoto T;Tatsukawa H;Hashimoto H;Hitomi K
钙依赖的谷氨酰胺转氨酶(TGS)是一类在多种生物体中催化蛋白质交联和/或伯胺结合的酶。哺乳动物的TGS参与多种生物学事件，如皮肤形成、血液凝固和细胞外基质稳定。青蒿鱼是研究哺乳动物蛋白质生理功能的一种模式鱼。通过对青竹基因组的分析，我们发现了7个TGS同源基因，其中一些显然与哺乳动物的TG同工酶TG1、TG2和因子XIII相对应。所有的同源物都在其推导的一级结构中保存了酶活性所必需的氨基酸残基。在这项研究中，我们分析了两个在转录水平上显著表达的哺乳动物上皮特异性TG(TG1)的同源基因(OlTGK1和OlTGK2)的生化特性。用纯化的OlTGK1和OlTGK2重组蛋白表征了它们的催化反应。此外，免疫组织化学FISH分析显示，在胰腺(OTGK1)、椎间盘(OlTGK2)和咽齿(OlTGK2)以及皮肤表皮中有较高的表达。
Calcium-dependent transglutaminases (TGs) are a family of enzymes that catalyze protein cross-linking and/or attachment of primary amines in a variety of organisms. Mammalian TGs are implicated in multiple biological events such as skin formation, blood coagulation, and extracellular matrix stabilization. Medaka (Oryzias latipes) has been used as a model fish to investigate the physiological functions of mammalian proteins. By analysis of the medaka genome, we found seven TGs orthologues, some of which apparently corresponded to the mammalian TG isozymes, TG1, TG2, and Factor XIII. All orthologues had preserved amino acid residues essential for enzymatic activity in their deduced primary structures. In this study, we analyzed biochemical properties of two orthologues (OlTGK1 and OlTGK2) of mammalian epithelium-specific TG (TG1) that are significantly expressed at the transcriptional level. Using purified recombinant proteins for OlTGK1 and OlTGK2, we characterized their catalytic reactions. Furthermore, immunohistochemical analyses of fish sections revealed higher expression in the pancreas (OTGK1), intervertebral disk (OlTGK2) and pharyngeal teeth (OlTGK2) as well as in the skin epidermis.