Comparative analysis of histone H3K27me3 modifications between blastocysts and somatic tissues in cattle
Comparative analysis of histone H3K27me3 modifications between blastocysts and somatic tissues in cattle
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DOI：
10.1111/asj.13684
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发表时间：
2022-01-01
影响因子：
2
通讯作者：
Minami, Naojiro
中科院分区：
文献类型：
--
作者：
Yamazaki, Satomi;Ikeda, Shuntaro;Minami, Naojiro
在早期发育阶段建立的表观遗传修饰可能对整个生命产生长期影响。这一概念包括在早期发育过程中通过表观遗传调控控制牲畜健康和疾病的可能性。为了探索早期胚胎中可能对成年期产生长期影响的表观遗传修饰，我们旨在获得牛囊胚的全基因组组蛋白H3赖氨酸27三甲基化（H3K27me3）谱，并将这些数据与成年体细胞组织的数据进行比较，以提取它们之间的共同和典型特征。体外制备牛囊胚，并对H3K27me3进行染色质免疫沉淀-测序分析。利用来自成人肌肉、脂肪和肝脏组织的公开数据对囊胚衍生的H3K27me3基因谱进行比较分析，发现(1)针对体细胞组织的囊胚特异性修饰在免疫功能相关基因中富集，(2)囊胚修饰“筛选”的体细胞修饰在组织特异性趋势的生物过程中富集。(3)胚泡和各体细胞组织中常见的修饰在发育重要基因（包括同源盒和印迹基因）中大量重叠和富集。本研究的结果产生了全基因组的牛囊胚H3K27me3图谱，并揭示了其与成人体细胞组织图谱的共同和典型特征。
Epigenetic modifications established in the early developmental stages can have long-term consequences throughout life. This concept encompasses the possibility of controlling livestock health and diseases by epigenetic regulation during early development. To explore the candidates of epigenetic modifications in early embryos that might exert long-lasting effects in adulthood, we aimed to obtain genome-wide histone H3 lysine 27 trimethylation (H3K27me3) profiles of bovine blastocysts and compare these data with those from adult somatic tissues in order to extract common and typical features between them. Bovine blastocysts were produced in vitro and subjected to chromatin immunoprecipitation-sequencing analysis of H3K27me3. Comparative analysis of the blastocyst-derived H3K27me3 profile performed using publicly available data from adult muscle, fat, and liver tissues revealed that (1) blastocyst-specific modifications against somatic tissues were enriched in immune function-related genes, (2) somatic modifications "sieved" by blastocyst modifications were enriched in biological processes in tissue-specific trends, (3) the modifications common in blastocyst and each somatic tissue were largely overlapped and enriched in developmentally important genes, including homeobox and imprinted genes. The results of this study produced a genome-wide H3K27me3 profile of bovine blastocysts and revealed its common and typical features in relation to the profiles of adult somatic tissues.