AGAR DERIVATIVES FOR CHROMATOGRAPHY, ELECTROPHORESIS AND GEL-BOUND ENZYMES .3. RIGID AGAROSE GELS CROSSLINKED WITH DIVINYL SULFONE (DVS)
AGAR DERIVATIVES FOR CHROMATOGRAPHY, ELECTROPHORESIS AND GEL-BOUND ENZYMES .3. RIGID AGAROSE GELS CROSSLINKED WITH DIVINYL SULFONE (DVS)
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DOI：
10.1016/s0021-9673(00)83800-5
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发表时间：
1975-01-01
期刊：
影响因子：
--
通讯作者：
JANSON, JC
中科院分区：
文献类型：
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作者：
PORATH, J;LAAS, T;JANSON, JC
二乙烯基砜交联琼脂糖 (DVS) 是一种用于色谱和固定化酶的新型基质，与普通琼脂糖凝胶相比具有明显的优势。与这些凝胶相比，它具有出色的医疗稳定性，并且刚性凝胶珠形成的床允许非常高的流速。此外，DVS-琼脂糖在酸性和中性介质中的化学稳定性优于琼脂糖凝胶。对于中度或高度交联的凝胶，在 pH 值高于 8 的碱性溶液中，含砜桥会缓慢消除，但在 pH 值低于 12 的情况下，凝胶不会出现明显的伴随溶解。 DVS-琼脂糖具有足够的热稳定性，可以在高压釜中加热。
Agarose cross-linked with divinyl sulphone (DVS) is a new matrix for chromatography and immobilized enzymes that has distinct advantages over common agarose gels. It has outstanding medical stability as compared with these gels, and the rigid gel beads form beds permitting very high flow-rates. In addition, DVS-agarose is superior to agarose gels with respect to chemical stability in acid and neutral media. In alkaline solutions above pH 8, there is a slow elimination of the sulphone-containing bridges, but without noticeable concomitant dissolution of the gels below pH 12 for moderately or highly cross-linked gels. The DVS-agarose is sufficiently thermostable to be heated in an autoclave.