Interaction of Fas(Apo-1/CD95) with proteins implicated in the ubiquitination pathway
Interaction of Fas(Apo-1/CD95) with proteins implicated in the ubiquitination pathway
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DOI：
10.1016/s0014-5793(97)00758-8
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发表时间：
1997-07-21
期刊：
影响因子：
3.5
通讯作者：
Tschopp, J
中科院分区：
文献类型：
--
作者：
Becker, K;Schneider, P;Tschopp, J
Fas(Apo-1/CD95)是属于肿瘤坏死因子受体家族的一种受体，当Fas配体触发时会诱导细胞凋亡。一旦激活，Fas的胞质结构域就会与多种传递死亡信号的蛋白质结合。我们使用酵母双杂交筛选来分离Fas相关蛋白，在这里我们报道泛素结合酶UBC9在Fas的死亡结构域和近膜区域之间的界面处与Fas结合，这种相互作用在体内也可见到。 UBC9 在 HeLa 细胞中瞬时表达，与 Fas 的共表达胞质片段结合。 FAF1 是一种增强细胞凋亡的 Fas 相关蛋白（Chu et al. (1996) Proc. Natl. Acad. Sci. USA 92, 11894-11898），被发现含有与泛素相似的序列。这些结果表明与泛素化途径相关的蛋白质可能调节 Fas 信号传导途径。 (C) 1997 年欧洲生化学会联合会。
Fas(Apo-1/CD95), a receptor belonging to the tumor necrosis factor receptor family, induces apoptosis when triggered by Fas ligand, Upon its activation, the cytoplasmic domain of Fas binds several proteins which transmit the death signal. We used the yeast two-hybrid screen to isolate Fas-associated proteins, Here we report that the ubiquitin-conjugating enzyme UBC9 binds to Fas at the interface between the death domain and the membrane-proximal region of Fas, This interaction is also seen in vivo. UBC9 transiently expressed in HeLa cells bound to the co-expressed cytoplasmic segment of Fas. FAF1, a Fas-associated protein that potentiates apoptosis (Chu et al. (1996) Proc. Natl. Acad. Sci. USA 92, 11894-11898), was found to contain sequences similar to ubiquitin, These results suggest that proteins related to the ubiquitination pathway may modulate the Fas signaling pathway. (C) 1997 Federation of European Biochemical Societies.