Angiotensin-(1-7) Treatment Restores Pancreatic Microcirculation Profiles A New Story in Acute Pancreatitis
Angiotensin-(1-7) Treatment Restores Pancreatic Microcirculation Profiles A New Story in Acute Pancreatitis
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血管紧张素 (1-7) 治疗可恢复胰腺微循环，揭示急性胰腺炎的新故事
DOI：
10.1097/mpa.0000000000001609
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发表时间：
2020
期刊：
影响因子：
2.9
通讯作者：
Yin Chenghong
中科院分区：
文献类型：
--
作者：
Wang Xueyan;Liu Mingming;Hu Weikai;Cui Tianyu;Yu Xiaozheng;Liu Ruixia;Yin Chenghong
目的探讨血管紧张素-(1-7)治疗对急性胰腺炎(AP)大鼠胰腺微血管运动和血液分布的影响，以及血管紧张素-(1-7)对胰腺微循环的影响。采用小鼠腹腔注射雨蛙素和脂多糖的方法诱导急性胰腺炎。胰腺炎经组织病理学、血清淀粉酶和高敏C反应蛋白证实。激光多普勒检测AP进展过程中胰腺微血管运动及血流分布情况。结果急性胰腺炎小鼠胰腺微循环呈病理性损伤，平均血流灌注量、相对血流速度、有效频率和微血管运动幅度均降低。Ang-(1-7)组小鼠胰腺病理损伤明显减轻。一贯，Ang-(1-7)治疗导致胰腺微循环特征的恢复。此外，非Ang-(1-7)治疗组小鼠胰腺微血管壁不规则、管腔狭窄、线粒体肿胀，Ang-(1-7)治疗可逆转这些超微结构损伤。血管紧张素-(1-7)可恢复胰腺微循环功能状态。
ObjectivesThe aim of this study was to investigate the changes of pancreatic microvascular vasomotion and blood distribution pattern in acute pancreatitis (AP), and whether Angiotensin (Ang)-(1–7) treatment could restore pancreatic microcirculation profiles.MethodsMice were randomly separated into control, AP, and Ang-(1–7)-treated AP (A-AP) group. Acute pancreatitis was induced in mice by intraperitoneal injection of cerulein and lipopolysaccharide. Pancreatitis was confirmed by histopathology, serum amylase, and high-sensitive C-reactive protein. Pancreatic microvascular vasomotion and blood distribution pattern in AP progression were assessed by laser Doppler. Meanwhile, ultrastructural changes of pancreatic microcirculation, including microvascular cavity and wall and endothelial mitochondria, were evaluated by transmission electron microscopy.ResultsAcute pancreatitis mice exhibited pathological pancreatic injuries with lower blood distribution pattern and decreased average blood perfusion, relative velocity, effective frequency, and amplitude of microvascular vasomotion. The pancreatic pathological injuries in Ang-(1–7)-treated mice were significantly alleviated. Consistently, Ang-(1–7) treatment led to a restoration in pancreatic microcirculation profiles. Furthermore, non–Ang-(1–7)-treated mice showed an irregular microvascular wall, narrow cavity, and swelling mitochondria, and these ultrastructural impairments were reversed by Ang-(1–7) administration.ConclusionsPancreatic microcirculation profiles are abnormal in the progression of AP. Angiotensin-(1–7) administration could restore functional status of pancreatic microcirculation.