Burkholderia multivorans requires species-specific GltJK for entry of a contact-dependent growth inhibition system protein.
Burkholderia multivorans requires species-specific GltJK for entry of a contact-dependent growth inhibition system protein.
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多食伯克霍尔德氏菌需要物种特异性的GltJK才能进入接触性生长抑制系统蛋白。
DOI：
10.1111/mmi.14783
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发表时间：
2021-09
影响因子：
3.6
通讯作者：
Garcia, Erin C.
中科院分区：
文献类型：
--
作者：
Myers-Morales, Tanya;Sim, Martha M. S.;DuCote, Tanner J.;Garcia, Erin C.
细菌间的相互拮抗和交流是微生物群落发展的驱动力。在许多革兰氏阴性菌中，接触性生长抑制(CDI)系统有助于这些微生物的相互作用。CDI系统在细胞−接触时将大表面暴露的蛋白质的有毒C末端传递到邻近细菌的细胞质中。被称为BCPA-CT的这种毒性效应区域的输入是由受体细胞外膜和内膜上的特定的、但在很大程度上未知的受体介导的。在这项研究中，我们证明了胞质膜蛋白GltJK是CDI系统蛋白BCPA-2进入多食伯克霍尔德氏菌受体细胞所必需的，GltJK是预测的ABC类型转运蛋白的组成部分。与目前的CDI模型一致，gltJK也是受体细胞对不同的BCPA-CT敏感所必需的，该BCPA-CT在预测的BCPA-2的“易位区域”内共享序列。引人注目的是，该易位区域与利用CDI的大肠杆菌GltJK系统蛋白的类似区域显示出较低的序列一致性。我们的结果表明，表达大肠杆菌gltJK的受体细菌对BCPA-2介导的细菌间拮抗具有抵抗力，这表明BCPA-2特异性识别Burkholderia GltJK。利用一系列嵌合蛋白，特异性决定簇被映射到GltK C末端的伯克霍尔德氏菌特异序列，从而深入了解BCPA在受体细胞膜上的转运。依赖接触的生长抑制系统通过向邻近细菌输送有毒效应物来介导细菌间的拮抗作用。本研究确定内膜蛋白GltJK可能是多毛伯克霍尔德氏菌CDI系统效应物BCPA-2的受体。该受体的特异性被映射到GltK的C-末端，表明该区域在BCPA-2跨越受体细菌细胞膜的转运中发挥重要作用。
Interbacterial antagonism and communication are driving forces behind microbial community development. In many Gram‐negative bacteria, contact‐dependent growth inhibition (CDI) systems contribute to these microbial interactions. CDI systems deliver the toxic C‐terminus of a large surface exposed protein to the cytoplasm of neighboring bacteria upon cell−contact. Termed the BcpA‐CT, import of this toxic effector domain is mediated by specific, yet largely unknown receptors on the recipient cell outer and inner membranes. In this study, we demonstrated that cytoplasmic membrane proteins GltJK, components of a predicted ABC‐type transporter, are required for entry of CDI system protein BcpA‐2 into Burkholderia multivorans recipient cells. Consistent with current CDI models, gltJK were also required for recipient cell susceptibility to a distinct BcpA‐CT that shared sequences within the predicted “translocation domain” of BcpA‐2. Strikingly, this translocation domain showed low sequence identity to the analogous region of an Escherichia coli GltJK‐utilizing CDI system protein. Our results demonstrated that recipient bacteria expressing E. coli gltJK were resistant to BcpA‐2‐mediated interbacterial antagonism, suggesting that BcpA‐2 specifically recognizes Burkholderia GltJK. Using a series of chimeric proteins, the specificity determinant was mapped to Burkholderia‐specific sequences at the GltK C‐terminus, providing insight into BcpA transport across the recipient cell cytoplasmic membrane. Contact‐dependent growth inhibition systems mediate inter‐bacterial antagonism by delivering toxic effectors to neighboring bacteria. This study identifies inner membrane proteins GltJK as a likely receptor for recipient cell import of a Burkholderia multivorans CDI system effector, BcpA‐2. Specificity of the receptor is mapped to the GltK C‐terminus, suggesting that this region plays an important role in the translocation of BcpA‐2 across the recipient bacterium cytoplasmic membrane.
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影响因子：
4.5
作者：
Anderson MS;Garcia EC;Cotter PA
通讯作者：
Cotter PA
影响因子：
6.4
作者：
Ruhe ZC;Wallace AB;Low DA;Hayes CS
通讯作者：
Hayes CS
影响因子：
3.7
作者：
Koskiniemi S;Garza-Sánchez F;Edman N;Chaudhuri S;Poole SJ;Manoil C;Hayes CS;Low DA
通讯作者：
Low DA
影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.15252/embj.201490546
发表时间：
2015-06-03
期刊：
The EMBO journal
影响因子：
--
作者：
Miyakoshi M;Chao Y;Vogel J
通讯作者：
Vogel J