A fast and inexpensive method for random spore analysis in Schizosaccharomyces pombe
A fast and inexpensive method for random spore analysis in Schizosaccharomyces pombe
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DOI：
10.1002/yea.1855
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发表时间：
2011-07-01
期刊：
影响因子：
2.6
通讯作者：
Singh, Jagmohan
中科院分区：
文献类型：
--
作者：
Khare, Asheesh K.;Singh, Balveer;Singh, Jagmohan
随机孢子分析是酵母遗传学的基本工具，用于确定基因连锁和通过遗传杂交产生重组后代。在实验上，它涉及用酶处理交配混合物，如酵母菌酶或溶胞酶，选择性地裂解营养细胞的细胞壁而不是孢子。在这里，我们描述了一种方法，即相对耐火性质的孢子处理与升高的温度和反复冻融促进随机孢子分析在低成本的裂殖酵母裂殖酵母粟酒裂殖酵母。由于芽殖酵母中孢子的相似性质，该方法应被证明对芽殖酵母和裂殖酵母中的随机孢子分析有用。版权所有（C）2011约翰威利父子有限公司
Random spore analysis is a fundamental tool of yeast genetics for determining gene linkage and the generation of recombinant progeny by genetic crosses. Experimentally it involves treatment of a mating mix with enzymes, such as zymolyase or lyticase, that selectively lyse the cell wall of vegetative cells rather than the spores. Here, we describe a method whereby the relative refractory nature of the spores to treatment with elevated temperature and repeated freeze-thawing facilitates random spore analysis at low cost in fission yeast Schizosaccharomyces pombe. Because of similar properties of spores in budding yeast, this method should prove to be useful for random spore analysis in both budding and fission yeasts. Copyright (C) 2011 John Wiley & Sons, Ltd.